Expression and activity of N-myristoyltransferase in lung inflammation of cattle and its role in neutrophil apoptosis

N-myristoyltransferase (NMT) attaches a 14 carbon fatty acid, myristic acid, to the N-terminal glycine residue of proteins. NMT exists in two isoforms NMT1 and NMT2. Myristoylated proteins play critical roles in protein-protein interactions, cell signaling and oncogenesis. Although elevated expressi...

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Bibliographic Details
Published in:Veterinary research (Paris) 2010-01, Vol.41 (1), p.9-9
Main Authors: SHRIVASTAV, Anuraag, SURI, Sarabjeet S, MOHR, Ryan, JANARDHAN, Kyathanahalli S, SHARMA, Rajendra K, SINGH, Baljit
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
Acyltransferases - metabolism
Animal biology
Animal genetics
Animals
Apoptosis - physiology
Biochemistry, Molecular Biology
Biological and medical sciences
Cattle
Cell Behavior
Cellular Biology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Enzymologic - physiology
Genetics
Immunohistochemistry
Immunology
Life Sciences
Lung - cytology
Lung - microbiology
Lung - pathology
Macrophages - enzymology
Male
Mannheimia haemolytica
Microbiology
Microbiology and Parasitology
Molecular biology
Neurons and Cognition
Neutrophils - enzymology
Original
Pneumonia of Calves, Enzootic - microbiology
Santé publique et épidémiologie
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Summary:N-myristoyltransferase (NMT) attaches a 14 carbon fatty acid, myristic acid, to the N-terminal glycine residue of proteins. NMT exists in two isoforms NMT1 and NMT2. Myristoylated proteins play critical roles in protein-protein interactions, cell signaling and oncogenesis. Although elevated expression of NMT1 has been described in colorectal carcinoma, its expression and roles in normal and inflamed lungs of the cattle are unknown. Therefore, we investigated the expression and activity of NMT in a bovine model of lung inflammation induced with Mannheimia hemolytica and in vitro in neutrophils and macrophages. Western blots revealed increased expression of NMT1 in lungs from infected animals compared to control animals. Total NMT activity was reduced in inflamed lungs compared to control animals (p < 0.05) along with increased expression of enolase, a putative inhibitor of NMT. NMT1 staining was observed in the septum, vascular endothelium and the epithelium in the lungs from control as well as infected calves. NMT1 expression was intense in neutrophils in the necrotic areas in the inflamed lungs. Immuno-electron microscopy localized NMT1 in cytoplasm and nuclei of endothelium, pulmonary intravascular macrophages and airway epithelium. Total NMT activity and NMT1 expression were increased in neutrophils and macrophages exposed to Escherichia coli LPS in vitro. NMT knockdown increased apoptosis in activated neutrophils. This is the first report demonstrating expression of NMT in normal and inflamed lungs and a novel role for NMT in regulation of neutrophil lifespan.
ISSN:0928-4249
1297-9716
DOI:10.1051/vetres/2009057