Spatiotemporal regulation of chloride intracellular channel protein CLIC4 by RhoA

• Published in: Molecular biology of the cell 2009-11, Vol.20 (22), p.4664-4672
• Main Authors: Ponsioen, Bas, van Zeijl, Leonie, Langeslag, Michiel, Berryman, Mark, Littler, Dene, Jalink, Kees, Moolenaar, Wouter H
• Format: Article
• Language: English
• Subjects: Actins - metabolism; Amino Acid Sequence; Animals; Cell Line; Cell Membrane - metabolism; Chloride Channels - genetics; Chloride Channels - metabolism; Cysteine - metabolism; Cytoskeleton - metabolism; DNA Mutational Analysis; GTP-Binding Protein alpha Subunits, G12-G13 - metabolism; Humans; Lysophospholipids - metabolism; Models, Molecular; Molecular Sequence Data; Patch-Clamp Techniques; Protein Structure, Tertiary; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; rhoA GTP-Binding Protein - metabolism; RNA Interference
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.e09-06-0529

Chloride intracellular channel (CLIC) 4 is a soluble protein structurally related to omega-type glutathione-S-transferases (GSTs) and implicated in various biological processes, ranging from chloride channel formation to vascular tubulogenesis. However, its function(s) and regulation remain unclear. Here, we show that cytosolic CLIC4 undergoes rapid but transient translocation to discrete domains at the plasma membrane upon stimulation of G(13)-coupled, RhoA-activating receptors, such as those for lysophosphatidic acid, thrombin, and sphingosine-1-phosphate. CLIC4 recruitment is strictly dependent on Galpha(13)-mediated RhoA activation and F-actin integrity, but not on Rho kinase activity; it is constitutively induced upon enforced RhoA-GTP accumulation. Membrane-targeted CLIC4 does not seem to enter the plasma membrane or modulate transmembrane chloride currents. Mutational analysis reveals that CLIC4 translocation depends on at least six conserved residues, including reactive Cys35, whose equivalents are critical for the enzymatic function of GSTs. We conclude that CLIC4 is regulated by RhoA to be targeted to the plasma membrane, where it may function not as an inducible chloride channel but rather by displaying Cys-dependent transferase activity toward a yet unknown substrate.