The Trehalose Pathway Regulates Mitochondrial Respiratory Chain Content through Hexokinase 2 and cAMP in Saccharomyces cerevisiae

In yeast, trehalose is synthesized by a multimeric enzymatic complex: TPS1 encodes trehalose 6-phosphate synthase, which belongs to a complex that is composed of at least three other subunits, including trehalose 6-phosphate phosphatase Tps2 and the redundant regulatory subunits Tps3 and Tsl1. The p...

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Bibliographic Details
Published in:The Journal of biological chemistry 2009-10, Vol.284 (40), p.27229-27234
Main Authors: Noubhani, Abdelmajid, Bunoust, Odile, Bonini, Beatriz Monge, Thevelein, Johan M., Devin, Anne, Rigoulet, Michel
Format: Article
Language:English
Subjects:
Cellular Biology
Cyclic AMP - metabolism
Cytochromes - metabolism
Electron Transport
Glucosyltransferases - genetics
Glucosyltransferases - metabolism
Hexokinase - metabolism
Kinetics
Life Sciences
Metabolism and Bioenergetics
Mitochondria - metabolism
Mutation
Phosphorylation
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Temperature
Trehalose - biosynthesis
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Summary:In yeast, trehalose is synthesized by a multimeric enzymatic complex: TPS1 encodes trehalose 6-phosphate synthase, which belongs to a complex that is composed of at least three other subunits, including trehalose 6-phosphate phosphatase Tps2 and the redundant regulatory subunits Tps3 and Tsl1. The product of the TPS1 gene plays an essential role in the control of the glycolytic pathway by restricting the influx of glucose into glycolysis. In this paper, we investigated whether the trehalose synthesis pathway could be involved in the control of the other energy-generating pathway: oxidative phosphorylation. We show that the different mutants of the trehalose synthesis pathway (tps1Δ, tps2Δ, and tps1,2Δ) exhibit modulation in the amount of respiratory chains, in terms of cytochrome content and maximal respiratory activity. Furthermore, these variations in mitochondrial enzymatic content are positively linked to the intracellular concentration in cAMP that is modulated by Tps1p through hexokinase2. This is the first time that a pathway involved in sugar storage, i.e. trehalose, is shown to regulate the mitochondrial enzymatic content.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M109.029454