An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate

Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from follow...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-12, Vol.390 (3), p.382-387
Main Authors: Tiwari, Vaibhav, Darmani, Nissar A., Thrush, Gerald R., Shukla, Deepak
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Cell Fusion
Cell Membrane - metabolism
Cell Membrane - virology
CHO Cells
Conjunctival epithelium
Cricetinae
Cricetulus
EPITHELIUM
Glycoprotein
GLYCOPROTEINS
Heparan sulfate
Heparitin Sulfate - metabolism
Herpesviridae Infections - metabolism
Herpesvirus 8, Human - metabolism
Human herpesvirus-8
HUMAN POPULATIONS
Humans
INHIBITION
Membrane Fusion
PATHOGENESIS
SULFATES
Viral Envelope Proteins - genetics
Viral Envelope Proteins - metabolism
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH–gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.08.174