Regulation of Meiotic Recombination via Mek1-Mediated Rad54 Phosphorylation

A preference for homologs over sister chromatids in homologous recombination is a fundamental difference in meiotic versus mitotic cells. In budding yeast, the bias for interhomolog recombination in meiosis requires the Dmc1 recombinase and the meiosis-specific kinase Mek1, which suppresses engageme...

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Bibliographic Details
Published in:Molecular cell 2009-11, Vol.36 (3), p.393-404
Main Authors: Niu, Hengyao, Wan, Lihong, Busygina, Valeria, Kwon, YoungHo, Allen, Jasmina A., Li, Xue, Kunz, Ryan C., Kubota, Kazuishi, Wang, Beatrice, Sung, Patrick, Shokat, Kevan M., Gygi, Steven P., Hollingsworth, Nancy M.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analogs & derivatives
Adenosine Triphosphate - metabolism
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Division
DNA
DNA Breaks, Double-Stranded
DNA Helicases
DNA Repair
DNA Repair Enzymes - genetics
DNA Repair Enzymes - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Immunoblotting
MAP Kinase Kinase 1 - genetics
MAP Kinase Kinase 1 - metabolism
Mass Spectrometry
Meiosis
Mutation
Phosphorylation
Protein Binding
Rad51 Recombinase - genetics
Rad51 Recombinase - metabolism
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Spores, Fungal - genetics
Threonine - metabolism
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Summary:A preference for homologs over sister chromatids in homologous recombination is a fundamental difference in meiotic versus mitotic cells. In budding yeast, the bias for interhomolog recombination in meiosis requires the Dmc1 recombinase and the meiosis-specific kinase Mek1, which suppresses engagement of sister chromatids by the mitotic recombinase Rad51. Here, a combination of proteomic, biochemical, and genetic approaches has identified an additional role for Mek1 in inhibiting the activity of the Rad51 recombinase through phosphorylation of its binding partner, Rad54. Rad54 phosphorylation of threonine 132 attenuates complex formation with Rad51, and a negative charge at this position reduces Rad51 function in vitro and in vivo. Thus, Mek1 phosphorylation provides a dynamic means of controlling recombination partner choice in meiosis in two ways: (1) it reduces Rad51 activity through inhibition of Rad51/Rad54 complex formation, and (2) it suppresses Rad51-mediated strand invasion of sister chromatids via a Rad54-independent mechanism.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2009.09.029