Identification of TRPM7 channels in human intestinal interstitial cells of Cajal

• Published in: World journal of gastroenterology : WJG 2009-12, Vol.15 (46), p.5799-5804
• Main Authors: Kim, Byung Joo, Park, Kyu Joo, Kim, Hyung Woo, Choi, Seok, Jun, Jae Yeoul, Chang, In Youb, Jeon, Ju-Hong, So, Insuk, Kim, Seon Jeong
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Boron Compounds - pharmacology; Female; Flufenamic Acid - pharmacology; Gastrointestinal Motility - physiology; Humans; Interstitial Cells of Cajal - cytology; Interstitial Cells of Cajal - metabolism; Intestines - cytology; Lanthanum - chemistry; Lanthanum - metabolism; Male; Middle Aged; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - metabolism; Original; Patch-Clamp Techniques; Protein-Serine-Threonine Kinases; TRPM Cation Channels - metabolism; 人体组织; 免疫组化; 国际商会; 离子通道; 胃肠道; 间质细胞; 非选择性
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.5799

AIM： To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 （TRPM7） in the human gastrointestinal （GI） tract. METHODS： Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajat （ICCs）. RESULTS： The human GI tract generated slow electrical waves and had ICCs which functioned as pacemak er cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB （2-aminoethoxydiphenyl borate） and La3＋, TRPM7 channel blockers, inhibited the slowwaves. Also, TRPM7 channels were expressed in ICCs in human tissue. CONCLUSION： These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the gen- eration of the slow waves.