Pleocytosis is associated with disruption of HIV compartmentalization between blood and cerebral spinal fluid viral populations

Abstract Introduction: We hypothesized that pleocytosis, which is a marker of central nervous system (CNS) inflammation, would result in viral genetic equilibration or de-compartmentalization between HIV populations in the blood and cerebrospinal fluid (CSF), suggesting viral trafficking. Methods :...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2009-03, Vol.385 (1), p.204-208
Main Authors: Smith, Davey M, Zárate, Selene, Shao, Hai, Pillai, Satish K, Letendre, Scott L, Wong, Joseph K, Richman, Douglas D, Frost, Simon D.W, Ellis, Ronald J
Format: Article
Language:English
Subjects:
Adult
Anti-HIV Agents - therapeutic use
Cerebrospinal fluid
Genetic Variation
HIV Infections - blood
HIV Infections - cerebrospinal fluid
HIV Infections - complications
HIV Infections - drug therapy
HIV Infections - virology
HIV Neurovirulence
HIV-1 - drug effects
HIV-1 - genetics
HIV-1 - isolation & purification
HIV-1 - physiology
Human immunodeficiency virus
Humans
Infectious Disease
Leukocytosis - blood
Leukocytosis - cerebrospinal fluid
Leukocytosis - etiology
Leukocytosis - genetics
Longitudinal Studies
Male
Middle Aged
Neuroadaptation
Phylogeny
Pleocytosis
Receptors, CCR5 - metabolism
RNA, Viral - blood
RNA, Viral - cerebrospinal fluid
Time Factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Introduction: We hypothesized that pleocytosis, which is a marker of central nervous system (CNS) inflammation, would result in viral genetic equilibration or de-compartmentalization between HIV populations in the blood and cerebrospinal fluid (CSF), suggesting viral trafficking. Methods : Study subjects, who started or interrupted their antiretroviral treatment, had viral loads measured and clonal viral env sequences generated from HIV RNA extracted from paired blood and CSF samples. White blood counts in CSF were also measured at each timepoint. Degree of inter-compartment segregation was calculated by posterior probability using linear discriminant analysis and multidimensional scaling. Co-receptor usage was determined using a trained support vector machine. Results : Pleocytosis was strongly associated with disruption of viral compartmentalization. Conclusions : Inflammation in the CNS, marked by pleocytosis, allows HIV populations to mix between blood and CSF, which may increase the overall viral genetic diversity within the CSF.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2008.11.010