Cell surface oligosaccharides participate in cohesion during aggregation of Dictyostelium discoideum

Plasma membrane glycoproteins from Dictyostelium discoideum amoebae at three stages of early development were digested with Pronase and endoglycosidase H and fractionated by gel filtration. This gave three classes of glycans (polysaccharides, endoglycosidase H-resistant glycopeptides, and endoglycos...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-02, Vol.85 (3), p.817-821
Main Authors: Ziska, S.E, Henderson, E.J
Format: Article
Language:English
Subjects:
Agglutination
AGGLUTINATION TESTS
Aggregation
AZUCARES
Biological and medical sciences
Cell aggregates
Cell Aggregation - drug effects
Cell interactions, adhesion
Cell membranes
CELL STRUCTURE
CELLS
CELLULE
CELULAS
CHAMPIGNON
Dictyostelium - physiology
Dictyostelium discoideum
ESTRUCTURA CELULAR
Fundamental and applied biological sciences. Psychology
Fungal Proteins - physiology
FUNGI
Gels
GLICOPROTEINAS
Glycopeptides
GLYCOPROTEINE
GLYCOPROTEINS
Glycoproteins - physiology
Membrane Proteins - physiology
Molecular and cellular biology
Oligosaccharides
Peptide Fragments - pharmacology
Polysaccharides
Polysaccharides - pharmacology
Polysaccharides - physiology
REACCIONES DE AGLUTINACION
REACTION D'AGGLUTINATION
Receptors
STRUCTURE CELLULAIRE
SUCRES
SUGARS
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Summary:Plasma membrane glycoproteins from Dictyostelium discoideum amoebae at three stages of early development were digested with Pronase and endoglycosidase H and fractionated by gel filtration. This gave three classes of glycans (polysaccharides, endoglycosidase H-resistant glycopeptides, and endoglycosidase H-released oligosaccharides), which were tested for their ability to block agglutination of amoebae from vegetative, aggregation (8-hr), and late-aggregation (13-hr) stages of development. The endoglycosidase H-resistant glycopeptides from 8-hr cells inhibited agglutination of disaggregated 8-hr cells but not vegetative or 13-hr cells. The 8-hr polysaccharide and endo H-sensitive oligosaccharides did not inhibit. The glycopeptides from 8-hr cells were resolved into five species by electrophoresis in boratecontaining buffer. Two of these had agglutination-inhibiting activity, and three did not. None of the glycan fractions from vegetative or 13-hr cells inhibited agglutination of vegetative, 8-, or 13-hr cells. These data implicate specific cell surface glycans in aggregation-stage intercellular cohesion and suggest that both these glycans and receptors for them are developmentally regulated.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.3.817