Predictive Computational Models of Substrate Binding by a Nucleoside Transporter

Transporters play a vital role in both the resistance mechanisms of existing drugs and effective targeting of their replacements. Melarsoprol and diamidine compounds similar to pentamidine and furamidine are primarily taken up by trypanosomes of the genus Trypanosoma brucei through the P2 aminopurin...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2009-12, Vol.284 (49), p.34028-34035
Main Authors: Collar, Catharine J., Al-Salabi, Mohammed I., Stewart, Mhairi L., Barrett, Michael P., Wilson, W.David, de Koning, Harry P.
Format: Article
Language:English
Subjects:
Animals
Chemistry, Pharmaceutical - methods
Computational Biology - methods
Computers
Drug Design
Female
Kinetics
Melarsoprol - pharmacology
Membrane Transport, Structure, Function, and Biogenesis
Nucleoside Transport Proteins - chemistry
Pentamidine - pharmacology
Rats
Rats, Wistar
Software
Trypanocidal Agents - chemistry
Trypanocidal Agents - pharmacology
Trypanosoma brucei
Trypanosoma brucei brucei - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transporters play a vital role in both the resistance mechanisms of existing drugs and effective targeting of their replacements. Melarsoprol and diamidine compounds similar to pentamidine and furamidine are primarily taken up by trypanosomes of the genus Trypanosoma brucei through the P2 aminopurine transporter. In standardized competition experiments with [3H]adenosine, P2 transporter inhibition constants (Ki) have been determined for a diverse dataset of adenosine analogs, diamidines, Food and Drug Administration-approved compounds and analogs thereof, and custom-designed trypanocidal compounds. Computational biology has been employed to investigate compound structure diversity in relation to P2 transporter interaction. These explorations have led to models for inhibition predictions of known and novel compounds to obtain information about the molecular basis for P2 transporter inhibition. A common pharmacophore for P2 transporter inhibition has been identified along with other key structural characteristics. Our model provides insight into P2 transporter interactions with known compounds and contributes to strategies for the design of novel antiparasitic compounds. This approach offers a quantitative and predictive tool for molecular recognition by specific transporters without the need for structural or even primary sequence information of the transport protein.
ISSN:0021-9258
1083-351X
1083-351X
DOI:10.1074/jbc.M109.049726