Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes

Purpose By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocys...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2009-12, Vol.26 (11-12), p.583-589
Main Authors: Liao, Hongqing, Zhang, Shuoping, Cheng, Dehua, OuYang, Qi, Lin, Ge, Gu, Yifan, Lu, Changfu, Gong, Fei, Lu, Guangxiu
Format: Article
Language:English
Subjects:
Blastocyst - cytology
Blastocyst - ultrastructure
Chi-Square Distribution
Chromosome Aberrations - embryology
Chromosomes, Human
Cytogenetic Analysis - methods
Embryonic Stem Cells - cytology
Embryonic Stem Cells - ultrastructure
Ethanol
Female
Fetuses
Genetics
Gynecology
Human Genetics
Humans
In Situ Hybridization, Fluorescence - methods
Male
Medicine
Medicine & Public Health
Ploidies
Pregnancy
Preimplantation Diagnosis - methods
Reproductive Medicine
Sex chromosomes
Sperm
Stem cells
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Summary:Purpose By comparing the chromosomal constitution among the arrested cleavage-stage embryos, blastocysts and human embryonic stem cells (hESCs) which are all derived from monopronuclear (1PN) zygotes, it is aimed to determine whether chromosomally normal embryos can be reliably selected by blastocyst culture. Methods After 1PN zygotes are sequentially cultured for 5 days, the blastocysts and arrested cleavage-stage embryos were analyzed by fluorescence in situ hybridization (FISH) with probes for chromosomes 18, X and Y; G-banding analysis was adopted to analyze the karyotype of 1PN hESCs. Results The diploid rate of blastocysts was 74.6%, which was significantly ( P  
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-009-9355-1