Nanoscale Cellular Changes in Field Carcinogenesis Detected by Partial Wave Spectroscopy

Understanding alteration of cell morphology in disease has been hampered by the diffraction-limited resolution of optical microscopy (>200 nm). We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell st...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2009-07, Vol.69 (13), p.5357-5363
Main Authors: SUBRAMANIAN, Hariharan, ROY, Hemant K, MOHAMMED, Jameel, CHANG, Jeen-Soo, BACKMAN, Vadim, PRADHAN, Prabhakar, GOLDBERG, Michael J, MULDOON, Joseph, BRAND, Randall E, STURGIS, Charles, HENSING, Thomas, RAY, Daniel, BOGOJEVIC, Andrej
Format: Article
Language:English
Subjects:
Adenoma - pathology
Antineoplastic agents
Biological and medical sciences
Carcinogenicity Tests
Cell Transformation, Neoplastic
Colon - cytology
Colon - pathology
Colonic Neoplasms - pathology
Duodenal Neoplasms - pathology
Duodenum - pathology
Gene Silencing
Humans
Intestinal Mucosa - cytology
Intestinal Mucosa - pathology
Medical sciences
Microarray Analysis - methods
MicroRNAs - genetics
Mutation
Pharmacology. Drug treatments
Precancerous Conditions - pathology
Sigmoidoscopy - methods
Spectrum Analysis - methods
Tumors
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Summary:Understanding alteration of cell morphology in disease has been hampered by the diffraction-limited resolution of optical microscopy (>200 nm). We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell structure at the nanoscale. Here we use PWS to show for the first time the increase in the disorder strength of the nanoscale architecture not only in tumor cells but also in the microscopically normal-appearing cells outside of the tumor. Although genetic and epigenetic alterations have been previously observed in the field of carcinogenesis, these cells were considered morphologically normal. Our data show organ-wide alteration in cell nanoarchitecture. This seems to be a general event in carcinogenesis, which is supported by our data in three types of cancer: colon, pancreatic, and lung. These results have important implications in that PWS can be used as a new method to identify patients harboring malignant or premalignant tumors by interrogating easily accessible tissue sites distant from the location of the lesion.
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.CAN-08-3895