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Nanoscale Cellular Changes in Field Carcinogenesis Detected by Partial Wave Spectroscopy
Understanding alteration of cell morphology in disease has been hampered by the diffraction-limited resolution of optical microscopy (>200 nm). We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell st...
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Published in: | Cancer research (Chicago, Ill.) Ill.), 2009-07, Vol.69 (13), p.5357-5363 |
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container_title | Cancer research (Chicago, Ill.) |
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creator | SUBRAMANIAN, Hariharan ROY, Hemant K MOHAMMED, Jameel CHANG, Jeen-Soo BACKMAN, Vadim PRADHAN, Prabhakar GOLDBERG, Michael J MULDOON, Joseph BRAND, Randall E STURGIS, Charles HENSING, Thomas RAY, Daniel BOGOJEVIC, Andrej |
description | Understanding alteration of cell morphology in disease has been hampered by the diffraction-limited resolution of optical microscopy (>200 nm). We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell structure at the nanoscale. Here we use PWS to show for the first time the increase in the disorder strength of the nanoscale architecture not only in tumor cells but also in the microscopically normal-appearing cells outside of the tumor. Although genetic and epigenetic alterations have been previously observed in the field of carcinogenesis, these cells were considered morphologically normal. Our data show organ-wide alteration in cell nanoarchitecture. This seems to be a general event in carcinogenesis, which is supported by our data in three types of cancer: colon, pancreatic, and lung. These results have important implications in that PWS can be used as a new method to identify patients harboring malignant or premalignant tumors by interrogating easily accessible tissue sites distant from the location of the lesion. |
doi_str_mv | 10.1158/0008-5472.CAN-08-3895 |
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We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell structure at the nanoscale. Here we use PWS to show for the first time the increase in the disorder strength of the nanoscale architecture not only in tumor cells but also in the microscopically normal-appearing cells outside of the tumor. Although genetic and epigenetic alterations have been previously observed in the field of carcinogenesis, these cells were considered morphologically normal. Our data show organ-wide alteration in cell nanoarchitecture. This seems to be a general event in carcinogenesis, which is supported by our data in three types of cancer: colon, pancreatic, and lung. These results have important implications in that PWS can be used as a new method to identify patients harboring malignant or premalignant tumors by interrogating easily accessible tissue sites distant from the location of the lesion.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>DOI: 10.1158/0008-5472.CAN-08-3895</identifier><identifier>PMID: 19549915</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Adenoma - pathology ; Antineoplastic agents ; Biological and medical sciences ; Carcinogenicity Tests ; Cell Transformation, Neoplastic ; Colon - cytology ; Colon - pathology ; Colonic Neoplasms - pathology ; Duodenal Neoplasms - pathology ; Duodenum - pathology ; Gene Silencing ; Humans ; Intestinal Mucosa - cytology ; Intestinal Mucosa - pathology ; Medical sciences ; Microarray Analysis - methods ; MicroRNAs - genetics ; Mutation ; Pharmacology. Drug treatments ; Precancerous Conditions - pathology ; Sigmoidoscopy - methods ; Spectrum Analysis - methods ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 2009-07, Vol.69 (13), p.5357-5363</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-af80275eff593203b8df123c673e1c4c43ab5a7c44fb23019350366db0fddcf53</citedby><cites>FETCH-LOGICAL-c591t-af80275eff593203b8df123c673e1c4c43ab5a7c44fb23019350366db0fddcf53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21884274$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19549915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SUBRAMANIAN, Hariharan</creatorcontrib><creatorcontrib>ROY, Hemant K</creatorcontrib><creatorcontrib>MOHAMMED, Jameel</creatorcontrib><creatorcontrib>CHANG, Jeen-Soo</creatorcontrib><creatorcontrib>BACKMAN, Vadim</creatorcontrib><creatorcontrib>PRADHAN, Prabhakar</creatorcontrib><creatorcontrib>GOLDBERG, Michael J</creatorcontrib><creatorcontrib>MULDOON, Joseph</creatorcontrib><creatorcontrib>BRAND, Randall E</creatorcontrib><creatorcontrib>STURGIS, Charles</creatorcontrib><creatorcontrib>HENSING, Thomas</creatorcontrib><creatorcontrib>RAY, Daniel</creatorcontrib><creatorcontrib>BOGOJEVIC, Andrej</creatorcontrib><title>Nanoscale Cellular Changes in Field Carcinogenesis Detected by Partial Wave Spectroscopy</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Understanding alteration of cell morphology in disease has been hampered by the diffraction-limited resolution of optical microscopy (>200 nm). We recently developed an optical microscopy technique, partial wave spectroscopy (PWS), which is capable of quantifying statistical properties of cell structure at the nanoscale. Here we use PWS to show for the first time the increase in the disorder strength of the nanoscale architecture not only in tumor cells but also in the microscopically normal-appearing cells outside of the tumor. Although genetic and epigenetic alterations have been previously observed in the field of carcinogenesis, these cells were considered morphologically normal. Our data show organ-wide alteration in cell nanoarchitecture. This seems to be a general event in carcinogenesis, which is supported by our data in three types of cancer: colon, pancreatic, and lung. These results have important implications in that PWS can be used as a new method to identify patients harboring malignant or premalignant tumors by interrogating easily accessible tissue sites distant from the location of the lesion.</description><subject>Adenoma - pathology</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Carcinogenicity Tests</subject><subject>Cell Transformation, Neoplastic</subject><subject>Colon - cytology</subject><subject>Colon - pathology</subject><subject>Colonic Neoplasms - pathology</subject><subject>Duodenal Neoplasms - pathology</subject><subject>Duodenum - pathology</subject><subject>Gene Silencing</subject><subject>Humans</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - pathology</subject><subject>Medical sciences</subject><subject>Microarray Analysis - methods</subject><subject>MicroRNAs - genetics</subject><subject>Mutation</subject><subject>Pharmacology. Drug treatments</subject><subject>Precancerous Conditions - pathology</subject><subject>Sigmoidoscopy - methods</subject><subject>Spectrum Analysis - methods</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNpVkF9LwzAUxYMoOqcfQcmLj9WkSdb0RZDqVJApqOhbuE2TGanpSOpg396UjalP90_OOTf8EDqh5JxSIS8IITITvMjPq6tZlnomS7GDRlQwmRWci1002moO0GGMn2kUlIh9dEBLwcuSihF6n4HvoobW4Mq07XcLAVcf4OcmYufx1Jm2wRUE7Xw3N95EF_G16Y3uTYPrFX6C0Dto8RssDX5epH1Icd1idYT2LLTRHG_qGL1Ob16qu-zh8fa-unrItChpn4GVJC-EsVaULCeslo2lOdOTghmqueYMagGF5tzWOSO0ZIKwyaSpiW0abQUbo8t17uK7_jKNNr4P0KpFcF8QVqoDp_6_ePeh5t1S5ekwLWQKEOsAnX4eg7FbLyVqQK0GjGrAqBJqlfoBdfKd_j3869qwTYKzjQAGwDaA1y5udTmVkucFZz8HZIkp</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>SUBRAMANIAN, Hariharan</creator><creator>ROY, Hemant K</creator><creator>MOHAMMED, Jameel</creator><creator>CHANG, Jeen-Soo</creator><creator>BACKMAN, Vadim</creator><creator>PRADHAN, Prabhakar</creator><creator>GOLDBERG, Michael J</creator><creator>MULDOON, Joseph</creator><creator>BRAND, Randall E</creator><creator>STURGIS, Charles</creator><creator>HENSING, Thomas</creator><creator>RAY, Daniel</creator><creator>BOGOJEVIC, Andrej</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20090701</creationdate><title>Nanoscale Cellular Changes in Field Carcinogenesis Detected by Partial Wave Spectroscopy</title><author>SUBRAMANIAN, Hariharan ; ROY, Hemant K ; MOHAMMED, Jameel ; CHANG, Jeen-Soo ; BACKMAN, Vadim ; PRADHAN, Prabhakar ; GOLDBERG, Michael J ; MULDOON, Joseph ; BRAND, Randall E ; STURGIS, Charles ; HENSING, Thomas ; RAY, Daniel ; BOGOJEVIC, Andrej</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c591t-af80275eff593203b8df123c673e1c4c43ab5a7c44fb23019350366db0fddcf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenoma - pathology</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Carcinogenicity Tests</topic><topic>Cell Transformation, Neoplastic</topic><topic>Colon - cytology</topic><topic>Colon - pathology</topic><topic>Colonic Neoplasms - pathology</topic><topic>Duodenal Neoplasms - pathology</topic><topic>Duodenum - pathology</topic><topic>Gene Silencing</topic><topic>Humans</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - pathology</topic><topic>Medical sciences</topic><topic>Microarray Analysis - methods</topic><topic>MicroRNAs - genetics</topic><topic>Mutation</topic><topic>Pharmacology. 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subjects | Adenoma - pathology Antineoplastic agents Biological and medical sciences Carcinogenicity Tests Cell Transformation, Neoplastic Colon - cytology Colon - pathology Colonic Neoplasms - pathology Duodenal Neoplasms - pathology Duodenum - pathology Gene Silencing Humans Intestinal Mucosa - cytology Intestinal Mucosa - pathology Medical sciences Microarray Analysis - methods MicroRNAs - genetics Mutation Pharmacology. Drug treatments Precancerous Conditions - pathology Sigmoidoscopy - methods Spectrum Analysis - methods Tumors |
title | Nanoscale Cellular Changes in Field Carcinogenesis Detected by Partial Wave Spectroscopy |
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