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Formation of the Preprimosome Protects λ O from RNA Transcription-Dependent Proteolysis by ClpP/ClpX

Using the bacteriophage λ DNA replication system, composed entirely of purified proteins, we have tested the accessibility of the short-lived λ O protein to the ClpP/ClpX protease during the various stages of λ DNA replication. We find that binding of λ O protein to its oriλ DNA sequence, leading to...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-12, Vol.95 (26), p.15259-15263
Main Authors: Zylicz, Maciej, Liberek, Krzysztof, Wawrzynow, Alicja, Georgopoulos, Costa
Format: Article
Language:English
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Summary:Using the bacteriophage λ DNA replication system, composed entirely of purified proteins, we have tested the accessibility of the short-lived λ O protein to the ClpP/ClpX protease during the various stages of λ DNA replication. We find that binding of λ O protein to its oriλ DNA sequence, leading to the so-called ``O-some'' formation, largely inhibits its degradation. On the contrary, under conditions permissive for transcription, the λ O protein bound to the oriλ sequence becomes largely accessible to ClpP/ClpX-mediated proteolysis. However, when the λ O protein is part of the larger oriλ :O· P· DnaB preprimosomal complex, transcription does not significantly increase ClpP/ClpX-dependent λ O degradation. These results show that transcription can stimulate proteolysis of a protein that is required for the initiation of DNA replication.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.26.15259