Kinesin 9 family members perform separate functions in the trypanosome flagellum

Numerous eukaryote genome projects have uncovered a variety of kinesins of unknown function. The kinesin 9 family is limited to flagellated species. Our phylogenetic experiments revealed two subfamilies: KIF9A (including Chlamydomonas reinhardtii KLP1) and KIF9B (including human KIF6). The function...

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Bibliographic Details
Published in:The Journal of cell biology 2009-11, Vol.187 (5), p.615-622
Main Authors: Demonchy, Raphaël, Blisnick, Thierry, Deprez, Caroline, Toutirais, Géraldine, Loussert, Céline, Marande, William, Grellier, Philippe, Bastin, Philippe, Kohl, Linda
Format: Article
Language:English
Subjects:
Antibodies
Cell extracts
Cell lines
Cell motility
Cytoskeleton
Cytoskeleton - metabolism
Eukaryotes
Flagella
Flagella - metabolism
Flagella - physiology
Flagella - ultrastructure
Genes
Genetic research
Genomics
Kinesin - analysis
Kinesin - genetics
Kinesin - physiology
Life Sciences
Microbiology and Parasitology
Microtubules
Organisms
Phenotypes
Phylogeny
Proteins
Protistology
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Protozoan Proteins - physiology
Trypanosoma brucei brucei - metabolism
Trypanosoma brucei brucei - ultrastructure
Trypanosome
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Summary:Numerous eukaryote genome projects have uncovered a variety of kinesins of unknown function. The kinesin 9 family is limited to flagellated species. Our phylogenetic experiments revealed two subfamilies: KIF9A (including Chlamydomonas reinhardtii KLP1) and KIF9B (including human KIF6). The function of KIF9A and KIF9B was investigated in the protist Trypanosoma brucei that possesses a single motile flagellum. KIF9A and KIF9B are strongly associated with the cytoskeleton and are required for motility. KIF9A is localized exclusively in the axoneme, and its depletion leads to altered motility without visible structural modifications. KIF9B is found in both the axoneme and the basal body, and is essential for the assembly of the paraflagellar rod (PFR), a large extra-axonemal structure. In the absence of KIF9B, cells grow abnormal flagella with excessively large blocks of PFR-like material that alternate with regions where only the axoneme is present. The functional diversity of the kinesin 9 family illustrates the capacity for adaptation of organisms to suit specific cytoskeletal requirements.
ISSN:0021-9525
1540-8140
DOI:10.1083/jcb.200903139