Verification by polymerase chain reaction of vertical transmission of Theileria sergenti in cows

To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) D...

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Bibliographic Details
Published in:Canadian journal of veterinary research 2003-10, Vol.67 (4), p.278-282
Main Authors: Baek, Byeong K, Soo, Kim B, Kim, Jin H, Hur, Jin, Lee, Bou O, Jung, J.M, Onuma, Misao, Oluoch, Anthony O, Kim, Chang-Hyun, Kakoma, Ibulaimu
Format: Article
Language:English
Subjects:
amino acids
Animals
blood
Blotting, Southern - veterinary
calves
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - parasitology
Cattle Diseases - transmission
cows
DNA
DNA Primers
DNA Probes
DNA, Protozoan - analysis
Female
fetus
herds
Infectious Disease Transmission, Vertical - veterinary
Open Reading Frames
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
polypeptides
Pregnancy
spleen
Theileria - genetics
Theileria - isolation & purification
Theileria sergenti
Theileriasis - diagnosis
Theileriasis - parasitology
Theileriasis - transmission
theileriosis
transplacental transmission
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Summary:To evaluate the transplacental transfer of Theileria sergenti infection in cattle, we used DNA probes to detect T. sergenti in 6 pregnant cows and their calves. All the animals were monitored by parasitologic, serologic, and polymerase chain reaction (PCR) assays for a predicted 875-base-pair (bp) DNA product and a 684-bp amplicon detected by nested PCR in the blood and spleens of aborted fetuses. An open reading frame (ORF) starting at nucleotide 170 and terminating at position 1021 was shown to code for a polypeptide of 283 amino acid residues. All 6 dams and 5 calves were positive for T. sergenti in all tests. One calf was positive only with nested PCR. We conclude that transplacental transmission of T. sergenti is a significant problem. The relevance of the data in the programmed introduction of new (especially pregnant) animals into established clean herds needs serious consideration with regard to control of theileriosis and other tickborne diseases.
ISSN:0830-9000