Expression of inducible nitric oxide synthase is increased in rat Barrett's esophagus induced by duodenal contents reflux

Barrett's esophagus is a premalignant condition of esophageal adenocarcinoma. Inducible nitric oxide synthase (iNOS) is induced by cytokines and can generate locally high concentrations of nitric oxide (NO), whose metabolites can mediate genotoxicity and influence multistage carcinogenesis by c...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Korean medical science 2005-02, Vol.20 (1), p.56-60
Main Authors: Bae, Jong Dae, Jung, Ki Hoon, Ahn, Woo Sup, Bae, Sung Han, Jang, Tae Jung
Format: Article
Language:English
Subjects:
Anastomosis, Surgical
Animals
Barrett Esophagus - enzymology
Barrett Esophagus - surgery
Cytoplasm - metabolism
Disease Models, Animal
DNA Damage
Duodenum - enzymology
Duodenum - surgery
Esophagus - metabolism
Immunohistochemistry
Male
Models, Anatomic
Neoplasms, Experimental - pathology
Nitric Oxide - metabolism
Nitric Oxide Synthase - biosynthesis
Nitric Oxide Synthase Type II
Original
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
RNA - metabolism
RNA, Messenger - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Barrett's esophagus is a premalignant condition of esophageal adenocarcinoma. Inducible nitric oxide synthase (iNOS) is induced by cytokines and can generate locally high concentrations of nitric oxide (NO), whose metabolites can mediate genotoxicity and influence multistage carcinogenesis by causing DNA damage. Therefore, we evaluated the immunolocalization and expression of iNOS in surgically induced rat Barrett's esophagus. Esophagoduodenal anastomosis was performed in rats for inducing reflux of duodenal contents. Rats were killed at postoperative 10, 20, 30 and 40 weeks. We examined histologic changes and iNOS expression in esophagus by immunohistochemistry and reverse transcription-polymerase chain reaction. Eighty six percent of experimental rats showed Barrett's esophagus above esophagoduodenal junction. iNOS immunoreactivity was clearly observed in the epithelial cells of Barrett's esophagus, predominantly at the apical surface of epithelial cells. Cytoplasmic staining was also seen only in atypical Barrett's esophagus. iNOS mRNA was detected only in the lower esophagus of experimental group. In conclusion, this study suggests that iNOS has some roles on Barrett's esophagus formation.
ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.2005.20.1.56