Origin and characteristics of enteroinvasive strains of Escherichia coli (EIEC) isolated in Germany

Thirty-five E. coli strains belonging to O-serogroups associated with enteroinvasive types of Escherichia coli (EIEC) isolated in Germany between 1989 and 1995 were investigated for invasivity-associated DNA sequences. Only 11 strains were positive for ipaH and thus confirmed as EIEC. All 11 EIEC is...

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Bibliographic Details
Published in:Epidemiology and infection 1997-06, Vol.118 (3), p.199-205
Main Authors: BEUTIN, L., GLEIER, K., KONTNY, I., ECHEVERRIA, P., SCHEUTZ, F.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Diarrhea
Diarrhea - microbiology
DNA Primers
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Epidemiology
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Escherichia coli Infections - microbiology
Europe
Fundamental and applied biological sciences. Psychology
Geographic regions
Germany
Humans
Infections
Microbiology
Nucleotide sequences
Pathogens
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Romania
Shigella
Travel
Virulence
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Summary:Thirty-five E. coli strains belonging to O-serogroups associated with enteroinvasive types of Escherichia coli (EIEC) isolated in Germany between 1989 and 1995 were investigated for invasivity-associated DNA sequences. Only 11 strains were positive for ipaH and thus confirmed as EIEC. All 11 EIEC isolates originated from human infections which were imported to Germany from Eastern Europe. EIEC O124 were most frequent and originated from asymptomatic Romanians arriving at Rostock, Germany in 1992 and 1993. In January 1993, EIEC O124 were isolated from faeces of a laboratory technician with diarrhoea working at the enteric pathogen department of the Institute of Hygiene in Rostock. By comparing her E. coli O124 isolate with recently imported O124 strains for Xba I restriction fragment length polymorphisms (RFLP) the probable source of infection could be determined. Four major RFLP patterns were found in the group of O124 strains. O124 strains with identical RFLP patterns were found in the group of O124 strains. O124 strains with identical RFLP patterns were isolated from people who were in close contact to each other.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268897007413