scaRNA2 is produced by an independent transcription unit and its processing is directed by the encoding region

The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase...

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Bibliographic Details
Published in:Nucleic acids research 2010-01, Vol.38 (2), p.370-381
Main Authors: Gérard, Marie-Aline, Myslinski, Evelyne, Chylak, Natassia, Baudrey, Stéphanie, Krol, Alain, Carbon, Philippe
Format: Article
Language:English
Subjects:
Binding Sites
Biochemistry, Molecular Biology
Cellular Biology
Gene Regulation, Chromatin and Epigenetics
HeLa Cells
Humans
Life Sciences
Promoter Regions, Genetic
RNA
RNA - biosynthesis
RNA - genetics
RNA - metabolism
RNA 3' End Processing
RNA Polymerase II
RNA Polymerase II - metabolism
RNA, Guide
RNA, Guide - biosynthesis
RNA, Guide - genetics
RNA, Guide - metabolism
Trans-Activators
Trans-Activators - metabolism
Transcription, Genetic
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Summary:The C/D box scaRNA2 is predicted to guide specific 2'-O-methylation of U2 snRNA. In contrast to other SCARNA genes, SCARNA2 appears to be independently transcribed. By transient expression of SCARNA2-reporter gene constructs, we have demonstrated that this gene is transcribed by RNA polymerase II and that the promoter elements responsible for its transcription are contained within a 161 bp region upstream of the transcription start site. In mammals, we have identified four cross species conserved promoter elements, a TATA motif, an hStaf/ZNF143 binding site and two novel elements that are required for full promoter activity. Binding of the human hStaf/ZNF143 transcription factor to its target sequence is required for promoter activity, suggesting that hStaf/ZNF143 is a fundamental regulator of the SCARNA2 gene. We also showed that RNA polymerase II continues transcription past the 3'-end of the mature RNA, irrespective of the identity of the Pol II promoter. The 3'-end processing and accumulation are governed by the sole information contained in the scaRNA2 encoding region, the maturation occurring via a particular pathway incompatible with that of mRNA or snRNA production.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkp988