Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli

• Published in: BioMed research international 2010-01, Vol.2010 (2010), p.1-6
• Main Authors: Pfrimer, Pollyana, Moraes, Lídia Maria Pepe de, Galdino, Alexsandro Sobreira, Salles, Loise P., Reis, Viviane Castelo Branco, De Marco, Janice Lisboa, Prates, Maura Vianna, Bloch, Carlos, Torres, Fernando Araripe Gonçalves
• Format: Article
• Language: English
• Subjects: Bacillus subtilis - enzymology; Bacteria; Biological and medical sciences; Cloning, Molecular; E coli; Enzyme Stability; Enzymes; Escherichia coli - metabolism; Hydrogen-Ion Concentration; Medical sciences; Microbiology; Pharmacology. Drug treatments; Proteins; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Time Factors; Urate Oxidase - chemistry; Urate Oxidase - genetics; Urate Oxidase - isolation & purification
• ISSN: 1110-7243; 2314-6133; 1110-7251; 2314-6141
• DOI: 10.1155/2010/674908

Urate oxidase (EC 1.7.3.3) is an enzyme involved in purine metabolism which is used in the treatment of gout and as diagnostic reagent for detection of uric acid. In order to produce this enzyme in large quantities for biotechnological purposes, the gene coding for the Bacillus subtilis urate oxidase was cloned and heterologously expressed in Escherichia coli. Time course induction in E. coli showed an induced protein with an apparent molecular mass of ∼60 kDa. Soluble recombinant enzyme was purified in a single-step procedure using Ni-NTA column. The enzyme was purified 2.1-fold with a yield of 56% compared to the crude extract. MALDI-TOF analysis revealed an ion with a mass of 58675 Da which is in agreement with the expected mass of the recombinant protein. The purified enzyme showed an optimal pH and temperature of 8.0 and 37∘C, respectively, and retained 90% of its activity after 72 hours of incubation at −20∘C and 4∘C.