Advances in Light Microscopy for Neuroscience

Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer...

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Bibliographic Details
Published in:Annual review of neuroscience 2009-01, Vol.32 (1), p.435-506
Main Authors: WILT, Brian A, BURNS, Laurie D, HO, Eric Tatt Wei, GHOSH, Kunal K, MUKAMEL, Eran A, SCHNITZER, Mark J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain
Fluorescence
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Humans
Image Cytometry - instrumentation
Image Cytometry - methods
Image Cytometry - trends
Mice
Mice, Transgenic
Microscopy
Microscopy - instrumentation
Microscopy - methods
Microscopy - trends
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Confocal - trends
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Microscopy, Fluorescence - trends
Molecular Biology - instrumentation
Molecular Biology - methods
Molecular Biology - trends
Nervous System - cytology
Neurons - cytology
Neurons - physiology
Neurosciences
Neurosciences - instrumentation
Neurosciences - methods
Neurosciences - trends
Rodents
Studies
Vertebrates: nervous system and sense organs
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Summary:Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer than the optical diffraction limit. Other progress permits functional imaging at faster speeds, at greater depths in brain tissue, and over larger tissue volumes than previously possible. Portable, miniaturized fluorescence microscopes now allow brain imaging in freely behaving mice. Complementary progress on animal preparations has enabled imaging in head-restrained behaving animals, as well as time-lapse microscopy studies in the brains of live subjects. Mouse genetic approaches permit mosaic and inducible fluorescence-labeling strategies, whereas intrinsic contrast mechanisms allow in vivo imaging of animals and humans without use of exogenous markers. This review surveys such advances and highlights emerging capabilities of particular interest to neuroscientists.
ISSN:0147-006X
1545-4126
DOI:10.1146/annurev.neuro.051508.135540