Sympathetic Nervous System Modulation of Inflammation and Remodeling in the Hypertensive Heart

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2010-02, Vol.55 (2), p.270-276
• Main Authors: Levick, Scott P, Murray, David B, Janicki, Joseph S, Brower, Gregory L
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Angiotensin II - metabolism; Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Cardiomegaly - pathology; Cardiomegaly - physiopathology; Cells, Cultured; Cytokines - metabolism; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Experimental diseases; Hypertension - pathology; Hypertension - physiopathology; Inflammation Mediators - analysis; Male; Medical sciences; Myocytes, Cardiac - cytology; Myocytes, Cardiac - physiology; Probability; Random Allocation; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Substance P - pharmacology; Sympathectomy; Sympathetic Nervous System - physiology; Ventricular Remodeling - physiology
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/HYPERTENSIONAHA.109.142042

Chronic activation of the sympathetic nervous system is a key component of cardiac hypertrophy and fibrosis. However, previous studies have provided evidence that also implicate inflammatory cells, including mast cells (MCs), in the development of cardiac fibrosis. The current study investigated the potential interaction of cardiac MCs with the sympathetic nervous system. Eight-week–old male spontaneously hypertensive rats were sympathectomized to establish the effect of the sympathetic nervous system on cardiac MC density, myocardial remodeling, and cytokine production in the hypertensive heart. Age-matched Wistar Kyoto rats served as controls. Cardiac fibrosis and hypertension were significantly attenuated and left ventricular mass normalized, whereas cardiac MC density was markedly increased in sympathectomized spontaneously hypertensive rats. Sympathectomy normalized myocardial levels of interferon-γ, interleukin 6, and interleukin 10, but had no effect on interleukin 4. The effects of norepinephrine and substance P on isolated cardiac MC activation were investigated as potential mechanisms of interaction between the two. Only substance P elicited MC degranulation. Substance P was also shown to induce the production of angiotensin II by a mixed population of isolated cardiac inflammatory cells, including MCs, lymphocytes, and macrophages. These results demonstrate the ability of neuropeptides to regulate inflammatory cell function, providing a potential mechanism by which the sympathetic nervous system and afferent nerves may interact with inflammatory cells in the hypertensive heart.