Improved reproducibility in preparing precision-cut liver tissue slices

Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since ther...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2009-12, Vol.61 (3), p.145-152
Main Authors: Zimmermann, Martina, Lampe, Johanna, Lange, Sebastian, Smirnow, Irina, Königsrainer, Alfred, Hann-von-Weyhern, Claus, Fend, Falko, Gregor, Michael, Bitzer, Michael, Lauer, Ulrich M
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biomedicine
Biotechnology
Cancer therapies
Chemistry
Chemistry and Materials Science
Cold
Fundamental and applied biological sciences. Psychology
Liver
R&D
Research & development
Sectioning
Stainless steel
Technical Note
Transplants & implants
Tumors
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Summary:Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great variability in the results, we sought to compare the reproducibility of tissue slices generated using the newly developed Leica VT1200 S vibrating blade microtome with Vibrocheck (LV) and the Krumdieck tissue slicer (KD) which has been the standard apparatus for this application so far. Liver samples from five different species (human, pig, cattle, rat, mouse) were cut and the reproducibility of slice thickness was analyzed by cross sectioning the PCLS. The quality of the sliced tissue was determined via measurement of the ATP content. As a result, we found an improved accuracy and reproducibility of rat, mouse and human tissue slices using the new Leica vibrating blade microtome.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-009-9246-4