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Isolation and characterization of a novel 2-sec-butylphenol-degrading bacterium Pseudomonas sp. strain MS-1
A novel bacterium capable of utilizing 2- sec -butylphenol as the sole carbon and energy source, Pseudomonas sp. strain MS-1, was isolated from freshwater sediment. Within 30 h, strain MS-1 completely degraded 1.5 mM 2- sec -butylphenol in basal salt medium, with concomitant cell growth. A pathway f...
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Published in: | Biodegradation (Dordrecht) 2010-04, Vol.21 (2), p.157-165 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A novel bacterium capable of utilizing 2-
sec
-butylphenol as the sole carbon and energy source,
Pseudomonas
sp. strain MS-1, was isolated from freshwater sediment. Within 30 h, strain MS-1 completely degraded 1.5 mM 2-
sec
-butylphenol in basal salt medium, with concomitant cell growth. A pathway for the metabolism of 2-
sec
-butylphenol by strain MS-1 was proposed on the basis of the identification of 3 internal metabolites—3-
sec
-butylcatechol, 2-hydroxy-6-oxo-7-methylnona-2,4-dienoic acid, and 2-methylbutyric acid—by gas chromatography-mass spectrometry analysis. Strain MS-1 degraded 2-
sec
-butylphenol through 3-
sec
-butylcatechol along a
meta
-cleavage pathway. Degradation experiments with various alkylphenols showed that the degradability of alkylphenols by strain MS-1 depended strongly on the position (
ortho
≫
meta
=
para
) of the alkyl substitute, and that strain MS-1 could degrade 2-alkylphenols with various sized and branched alkyl chain (
o
-cresol, 2-ethylphenol, 2-
n
-propylphenol, 2-isopropylphenol, 2-
sec
-butylphenol, and 2-
tert
-butylphenol), as well as a dialkylphenol (namely, 6-
tert
-butyl-
m
-cresol). |
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ISSN: | 0923-9820 1572-9729 |
DOI: | 10.1007/s10532-009-9290-y |