Advances in imaging of new targets for pharmacological intervention in stroke: real‐time tracking of T‐cells in the ischaemic brain

• Published in: British journal of pharmacology 2010-02, Vol.159 (4), p.808-811
• Main Authors: Ortolano, F, Maffia, P, Dever, G, Rodolico, G, Millington, OR, De Simoni, MG, Brewer, JM, Bushell, TJ, Garside, P, Carswell, HV
• Format: Article
• Language: English
• Subjects: Adoptive Transfer; Animals; Biological and medical sciences; Brain - immunology; Brain - metabolism; brain imaging; Disease Models, Animal; Feasibility Studies; Female; Fluoresceins - metabolism; Fluorescent Dyes - metabolism; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Infarction, Middle Cerebral Artery - immunology; Infarction, Middle Cerebral Artery - metabolism; inflammation; leukocytes; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microscopy, Fluorescence, Multiphoton; middle cerebral artery occlusion; Molecular Imaging; Molecular Probe Techniques; multiphoton microscopy; Neurology; Pharmacology. Drug treatments; stroke; Succinimides - metabolism; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; T-Lymphocytes - transplantation; Themed Section: Research Papers; Time Factors; Vascular diseases and vascular malformations of the nervous system
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.2009.00527.x

Background and purpose:  T‐cells may play a role in the evolution of ischaemic damage and repair, but the ability to image these cells in the living brain after a stroke has been limited. We aim to extend the technique of real‐time in situ brain imaging of T‐cells, previously shown in models of immunological diseases, to models of experimental stroke. Experimental approach:  Male C57BL6 mice (6–8 weeks) (n= 3) received a total of 2–5 × 106 carboxyfluorescein diacetate succinimidyl ester (CFSE)‐labelled lymphocytes from donor C57BL6 mice via i.v. injection by adoptive transfer. Twenty‐four hours later, recipient mice underwent permanent left distal middle cerebral artery occlusion (MCAO) by electrocoagulation or by sham surgery under isoflurane anaesthesia. Female hCD2‐green fluorescent protein (GFP) transgenic mice that exhibit GFP‐labelled T‐cells underwent MCAO. At 24 or 48 h post‐MCAO, a sagittal brain slice (1500 µm thick) containing cortical branches of the occluded middle cerebral artery (MCA) was dissected and used for multiphoton laser scanning microscopy (MPLSM). Key results:  Our results provide direct observations for the first time of dynamic T‐cell behaviour in living brain tissue in real time and herein proved the feasibility of MPLSM for ex vivo live imaging of immune response after experimental stroke. Conclusions and Implications:  It is hoped that these advances in the imaging of immune cells will provide information that can be harnessed to a therapeutic advantage. This article is part of a themed section on Imaging in Pharmacology. To view the editorial for this themed section visit http://dx.doi.org/10.1111/j.1476‐5381.2010.00685.x