Trypanosoma cruzi Lineages Detected in Congenitally Infected Infants and Triatoma infestans from the Same Disease-Endemic Region under Entomologic Surveillance in Paraguay

Trypanosoma cruzi II is associated with Chagas disease in the southern part of South America. We analyzed T. cruzi variants in field-collected triatomines and congenitally infected infants living in the same disease-endemic region in Paraguay. Results of polymerase chain reactions for T. cruzi kinet...

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Bibliographic Details
Published in:The American journal of tropical medicine and hygiene 2010-03, Vol.82 (3), p.386-390
Main Authors: Puerto, Florencia del, Sanchez, Zunilda, Nara, Eva, Meza, Graciela, Paredes, Berta, Ferreira, Elizabeth, Russomando, Graciela
Format: Article
Language:English
Subjects:
Adult
Animals
Biological and medical sciences
Chagas Disease - epidemiology
Chagas Disease - parasitology
Child, Preschool
Endemic Diseases
Female
Humans
Infant
Infectious Disease Transmission, Vertical
Infectious diseases
Male
Medical sciences
Paraguay - epidemiology
Polymerase Chain Reaction
Pregnancy
RNA, Protozoan - genetics
RNA, Ribosomal - genetics
Sensitivity and Specificity
Species Specificity
Triatoma - parasitology
Trypanosoma cruzi - genetics
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Summary:Trypanosoma cruzi II is associated with Chagas disease in the southern part of South America. We analyzed T. cruzi variants in field-collected triatomines and congenitally infected infants living in the same disease-endemic region in Paraguay. Results of polymerase chain reactions for T. cruzi kinetoplast DNA and satellite DNA were positive in 83 triatomine feces samples and 58 infant blood samples. However, lineages were detected in 33 and 38 samples, respectively. Trypanosoma cruzi genotypes were determined in 56 (97%) blood samples after hybridization by using specific probes. The Tc I genotype was not detected. The prevalent sublineage was Tc IId in triatomines (27 of 33) and infant blood (36 of 58) as assessed by amplification of the 24Salpha ribosomal RNA and the mini-exon region genes. The Tc IIc genotype was detected in 20 infant blood samples and in 1 triatomine. This study shows T. cruzi II is the predominant lineage circulating in triatomines and humans in endemic areas of eastern region of Paraguay.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2010.09-0006