Deficient Photosystem II in Agranal Bundle Sheath Chloroplasts of C4 Plants

A method is described for separating mesophyll and bundle sheath chloroplasts from the leaves of C4 plants. The agranal bundle sheath chloroplasts are inactive in the Hill reaction, whereas granal bundle sheath and granal mesophyll chloroplasts exhibit normal photosystem II activity. The agranal bun...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1970-09, Vol.67 (1), p.18-25
Main Authors: Woo, K. C., Anderson, Jan M., Boardman, N. K., Downton, W. J. S., Osmond, C. B., Thorne, S. W.
Format: Article
Language:English
Subjects:
Biological Sciences: Botany
Bundle sheath cells
Chloroplasts
Cytochromes
Fluorescence
Grana
Mesophyll
Photosystem I
Photosystem II
Sorghum
Wavelengths
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Summary:A method is described for separating mesophyll and bundle sheath chloroplasts from the leaves of C4 plants. The agranal bundle sheath chloroplasts are inactive in the Hill reaction, whereas granal bundle sheath and granal mesophyll chloroplasts exhibit normal photosystem II activity. The agranal bundle sheath chloroplasts are deficient in photosystem II; they lack cytochrome b-559 and the fluorescence bands associated with photosystem II. All the chloroplasts exhibit photosystem I activity.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.67.1.18