Delivery of Therapeutic Proteins as Secretable TAT Fusion Products

• Published in: Molecular therapy 2009-02, Vol.17 (2), p.334-342
• Main Authors: Flinterman, Marcella, Farzaneh, Farzin, Habib, Nagy, Malik, Farooq, Gäken, Joop, Tavassoli, Mahvash
• Format: Article
• Language: English
• Subjects: Amino acids; Bacteria; Blotting, Western; Cell death; Cell Line; Cloning; Endoplasmic reticulum; Enzymes; Flow Cytometry; Furin - genetics; Furin - metabolism; Gene therapy; Genetic Therapy - methods; Genetic Vectors - genetics; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Microscopy; Microscopy, Confocal; Microscopy, Fluorescence; Mutation; Oncology; Original; Peptides; Phosphorylation; Protein Structure, Tertiary; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; tat Gene Products, Human Immunodeficiency Virus - genetics; tat Gene Products, Human Immunodeficiency Virus - metabolism; Transduction, Genetic - methods
• ISSN: 1525-0016; 1525-0024
• DOI: 10.1038/mt.2008.256

The trans-acting activator of transcription (TAT) protein transduction domain (PTD) mediates the transduction of peptides and proteins into target cells. The TAT-PTD has an important potential as a tool for the delivery of therapeutic agents. The production of TAT fusion proteins in bacteria, however, is problematic because of protein insolubility and the absence of eukaryotic post-translational modification. An attractive alternative, both for in vitro protein production and for in vivo applications, is the use of higher eukaryotic cells for secretion of TAT fusion proteins. However, the ubiquitous expression of furin endoprotease (PACE or SPC1) in the Golgi/endoplasmic reticulum, and the presence of furin recognition sequences within TAT-PTD, results in the cleavage and loss of the TAT-PTD domain during its secretory transition through the endoplasmic reticulum and Golgi. In this study, we show the development of a synthetic TATκ-PTD in which mutation of the furin recognition sequences, but retention of protein transduction activity, allows secretion of recombinant proteins, followed by successful uptake of the modified protein, by the target cells. This system was used to successfully secrete marker protein, green fluorescent protein (GFP), and apoptin, a protein with tumor-specific cytotoxicity. Detection of GFP, phosphorylation, and induction of cell death by TATκ-GFP-apoptin indicated that the secreted proteins were functional in target cells. This novel strategy therefore has important potential for the efficient delivery of therapeutic proteins.