Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques

There are currently no nonhuman primate models with fully defined major histocompatibility complex (MHC) class II genetics. We recently showed that six common MHC haplotypes account for essentially all MHC diversity in cynomolgus macaques (Macaca fascicularis) from the island of Mauritius. In this s...

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Bibliographic Details
Published in:Immunogenetics (New York) 2007-06, Vol.59 (6), p.449-462
Main Authors: O'Connor, Shelby L, Blasky, Alex J, Pendley, Chad J, Becker, Ericka A, Wiseman, Roger W, Karl, Julie A, Hughes, Austin L, O'Connor, David H
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Cloning
Cynomolgus
Genes, MHC Class II
Genetic Variation
Genetics
Haplotypes
Humans
immunogenetics
Lymphocytes
Macaca fascicularis
Macaca fascicularis - genetics
Macaca mulatta
MHC
Molecular Sequence Data
Phylogeny
Primates
Reagents
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Summary:There are currently no nonhuman primate models with fully defined major histocompatibility complex (MHC) class II genetics. We recently showed that six common MHC haplotypes account for essentially all MHC diversity in cynomolgus macaques (Macaca fascicularis) from the island of Mauritius. In this study, we employ complementary DNA cloning and sequencing to comprehensively characterize full length MHC class II alleles expressed at the Mafa-DPA, -DPB, -DQA, -DQB, -DRA, and -DRB loci on the six common haplotypes. We describe 34 full-length MHC class II alleles, 12 of which are completely novel. Polymorphism was evident at all six loci including DPA, a locus thought to be monomorphic in rhesus macaques. Similar to other Old World monkeys, Mauritian cynomolgus macaques (MCM) share MHC class II allelic lineages with humans at the DQ and DR loci, but not at the DP loci. Additionally, we identified extensive sharing of MHC class II alleles between MCM and other nonhuman primates. The characterization of these full-length-expressed MHC class II alleles will enable researchers to generate MHC class II transferent cell lines, tetramers, and other molecular reagents that can be used to explore CD4+ T lymphocyte responses in MCM.
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-007-0209-7