Resistance Exercise, Skeletal Muscle FOXO3A, and 85-Year-Old Women

This investigation examined Akt-FOXO3A signaling in young women (YW) and old women (OW) before and after 12 weeks of high-intensity resistance training. Muscle biopsies were taken from the vastus lateralis before and immediately after resistance exercise (RE) in the untrained and trained states. In...

Full description

Saved in:
Bibliographic Details
Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2010-04, Vol.65A (4), p.335-343
Main Authors: Williamson, David L., Raue, Ulrika, Slivka, Dustin R., Trappe, Scott
Format: Article
Language:English
Subjects:
Aged, 80 and over
Aging - physiology
Blotting, Western
Cells
Exercise
Female
Forkhead Box Protein O3
Forkhead Transcription Factors - analysis
Forkhead Transcription Factors - genetics
Gene Expression
Gerontology
Humans
Journal of Gerontology: BIOLOGICAL SCIENCES
Muscle, Skeletal - chemistry
Musculoskeletal system
Oldest old people
Polymerase Chain Reaction
Resistance Training
Reverse Transcriptase Polymerase Chain Reaction
Signal transduction
Signal Transduction - physiology
Women
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This investigation examined Akt-FOXO3A signaling in young women (YW) and old women (OW) before and after 12 weeks of high-intensity resistance training. Muscle biopsies were taken from the vastus lateralis before and immediately after resistance exercise (RE) in the untrained and trained states. In response to RE in YW and OW, phospho Akt Thr308 increased in untrained and trained states, with no change on Ser473 site. FOXO3A-Ser253 site was dephosphorylated in untrained state among YW and OW, and nuclear phospho-FOXO3A increased mainly in YW in trained state. In the basal state, OW displayed lower cytosolic phospho-FOXO3A before training, higher total nuclear FOXO3A, and a trend for higher nuclear-to-cytosolic FOXO3A ratio versus YW after 12 weeks. Basal level MuRF-1 and myostatin mRNA decreased in YW, while OW increased myostatin mRNA after 12-weeks. These data suggest that FOXO3A signaling and FOXO3A-related target gene expression are altered in OW and may partially explain the attenuated training adaptations previously reported in these octogenarian women.
ISSN:1079-5006
1758-535X
DOI:10.1093/gerona/glq005