Characterization of hydrogen peroxide production by Duox in bronchial epithelial cells exposed to Pseudomonas aeruginosa

Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Ps...

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Bibliographic Details
Published in:FEBS letters 2010-03, Vol.584 (5), p.917-922
Main Authors: Rada, Balázs, Leto, Thomas L.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
airway surface liquid
air–liquid interface
ALI
ASL
Bacterial Proteins - genetics
Bacterial Proteins - physiology
Blotting, Western
Burkholderia cepacia
Burkholderia cepacia - physiology
Calcium - metabolism
Cell Line
CFTR
cystic fibrosis
cystic fibrosis transmembrane conductance regulator
diphenylene iodonium
DPI
Dual oxidase
Dual Oxidases
Duox
Epithelial cell
Epithelial Cells - metabolism
Epithelial Cells - microbiology
H2O2
horse-radish peroxidase
HRP
HTBE
human tracheobronchial epithelial cells
Humans
Hydrogen peroxide
Hydrogen Peroxide - metabolism
hypothiocyanite
lipopolysaccharide
LPS
NADPH oxidase
NADPH Oxidases - genetics
NADPH Oxidases - metabolism
Nox
OSCN
Pseudomonas
Pseudomonas aeruginosa
Pseudomonas aeruginosa - metabolism
Pseudomonas aeruginosa - physiology
relative luminescence unit
Reverse Transcriptase Polymerase Chain Reaction
reverse transcription
RLU
RNA Interference
SCN
Staphylococcus aureus
Staphylococcus aureus - physiology
T3SS
thiocyanate
TLR
toll-like receptor
type-three secretion system
Virulence Factors - genetics
Virulence Factors - physiology
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Description
Summary:Hydrogen peroxide production by the NADPH oxidase Duox1 occurs during activation of respiratory epithelial cells stimulated by purified bacterial ligands, such as lipopolysaccharide. Here, we characterize Duox activation using intact bacterial cells of several airway pathogens. We found that only Pseudomonas aeruginosa, not Burkholderia cepacia or Staphylococcus aureus, triggers H 2O 2 production in bronchial epithelial cells in a calcium-dependent but predominantly ATP-independent manner. Moreover, by comparing mutant Pseudomonas strains, we identify several virulence factors that participate in Duox activation, including the type-three secretion system. These data provide insight on Duox activation by mechanisms unique to P. aeruginosa.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2010.01.025