Quantitative Proteomics Analysis of Chondrogenic Differentiation of C3H10T1/2 Mesenchymal Stem Cells by iTRAQ Labeling Coupled with On-line Two-dimensional LC/MS/MS

The chondrogenic potential of multipotent mesenchymal stem cells (MSCs) makes them a promising source for cell-based therapy of cartilage defects; however, the exact intracellular molecular mechanisms of chondrogenesis as well as self-renewal of MSCs remain largely unknown. To gain more insight into...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2010-03, Vol.9 (3), p.550-564
Main Authors: Ji, Yu-hua, Ji, Ju-ling, Sun, Fen-yong, Zeng, Yao-ying, He, Xian-hui, Zhao, Jing-xian, Yu, Yu, Yu, Shou-he, Wu, Wei
Format: Article
Language:English
Subjects:
Animals
Bone Morphogenetic Protein 2 - metabolism
Cartilage - metabolism
Cell Differentiation
Cell Line
Chondrocytes - chemistry
Chondrocytes - cytology
Chondrocytes - metabolism
Chondrogenesis
Chromatography, Liquid
Down-Regulation
Extracellular Matrix - metabolism
Extracellular Matrix Proteins - genetics
Internet
Mesenchymal Stem Cells - chemistry
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mice
Proteomics
Recombinant Proteins - genetics
RNA, Messenger - genetics
Tandem Mass Spectrometry
Transcription Factors - genetics
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Summary:The chondrogenic potential of multipotent mesenchymal stem cells (MSCs) makes them a promising source for cell-based therapy of cartilage defects; however, the exact intracellular molecular mechanisms of chondrogenesis as well as self-renewal of MSCs remain largely unknown. To gain more insight into the underlying molecular mechanisms, we applied isobaric tag for relative and absolute quantitation (iTRAQ) labeling coupled with on-line two-dimensional LC/MS/MS technology to identify proteins differentially expressed in an in vitro model for chondrogenesis: chondrogenic differentiation of C3H10T1/2 cells, a murine embryonic mesenchymal cell line, was induced by micromass culture and 100 ng/ml bone morphogenetic protein 2 treatment for 6 days. A total of 1756 proteins were identified with an average false discovery rate
ISSN:1535-9476
1535-9484
1535-9484
DOI:10.1074/mcp.M900243-MCP200