Identification and characterization of subpopulations of Pax3 and Pax7 expressing cells in developing chick somites and limb buds

Pax3 and Pax7 are closely related paired‐boxed family transcription factors that are known to play important roles in embryonic and adult myogenesis. Previous reports describing the expression of Pax3 and Pax7 transcripts reveal expression in many overlapping domains. In this manuscript, we extend t...

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Bibliographic Details
Published in:Developmental dynamics 2008-07, Vol.237 (7), p.1862-1874
Main Authors: Galli, Lisa M., Knight, Sara R., Barnes, Tiffany L., Doak, Allison K., Kadzik, Rachel S., Burrus, Laura W.
Format: Article
Language:English
Subjects:
Animals
BrdU
Cell Proliferation
chick
Chick Embryo
Chickens
dermomyotome
Immunohistochemistry
limb
Limb Buds - cytology
Limb Buds - embryology
Limb Buds - metabolism
Microscopy, Confocal
MyoD
myogenesis
Myogenin
myotome
Paired Box Transcription Factors - biosynthesis
Pax3
Pax7
PAX7 Transcription Factor - biosynthesis
phosphohistone H3
somite
Somites - cytology
Somites - embryology
Somites - metabolism
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Summary:Pax3 and Pax7 are closely related paired‐boxed family transcription factors that are known to play important roles in embryonic and adult myogenesis. Previous reports describing the expression of Pax3 and Pax7 transcripts reveal expression in many overlapping domains. In this manuscript, we extend these studies by examining the protein expression profiles for Pax3 and Pax7 in developing chick somites and limbs with cellular resolution. Our studies show the existence of distinct subpopulations of cells in the somite and developing limb that are defined by the relative expression levels of Pax3 and Pax7. We also show that Pax3 and Pax7 negatively regulate each other's expression in the dermomyotome, thus providing a possible mechanism for the maintenance of observed expression patterns in the dermomyotome. Further characterization of Pax3‐ and/or Pax7‐positive cells in the dermomyotome and myotome with respect to proliferation and differentiation reveals subpopulations of cells with distinct properties. Developmental Dynamics 237:1862–1874, 2008. © 2008 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21585