Trypanosoma cruzi-specific immune responses in subjects from endemic areas of Chagas disease of Argentina

Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-γ ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-γ responses were detected in 42% of subjects evaluated (15/26 positive by conv...

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Bibliographic Details
Published in:Microbes and infection 2010-05, Vol.12 (5), p.359-363
Main Authors: Olivera, Gabriela C., Albareda, Maria C., Alvarez, Maria G., De Rissio, Ana M., Fichera, Laura E., Cooley, Gretchen, Yachelini, Pedro, Hrellac, Hugo A., Riboldi, Hilda, Laucella, Susana A., Tarleton, Rick L., Postan, Miriam
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Protozoan - blood
Argentina
Biological and medical sciences
Chagas Disease - immunology
Endemic Diseases
Female
Fundamental and applied biological sciences. Psychology
Human protozoal diseases
Humans
Humoral responses
Immunoblotting
Immunoglobulin G - blood
Infectious diseases
Interferon-gamma - secretion
Leukocytes, Mononuclear - immunology
Life cycle. Host-agent relationship. Pathogenesis
Male
Medical sciences
Microbiology
Middle Aged
Parasitic diseases
Protozoa
Protozoal diseases
T cell responses
Trypanosoma
Trypanosoma cruzi
Trypanosoma cruzi - immunology
Trypanosomiasis
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Summary:Trypanosoma cruzi-specific immune responses were evaluated in a total of 88 subjects living in areas endemic of Chagas disease of Argentina by IFN-γ ELISPOT assays and immunoblotting. Positive T. cruzi antigen-induced IFN-γ responses were detected in 42% of subjects evaluated (15/26 positive by conventional serology and 22/62 seronegative subjects). Using immunoblotting, T. cruzi-specific IgG reactivity was detected in all seropositive subjects and in 11% (7/61) of subjects negative by conventional serology. Measurements of T cell responses and antibodies by immunoblotting, in conjunction with conventional serology, might enhance the capability of detection of exposure to T. cruzi in endemic areas.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2010.01.011