RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids

Both natural and newly synthesized allopolyploids display nonadditive gene expression changes through genetic and epigenetic mechanisms. The nonadditively expressed genes include many microRNA (miRNA) targets, suggesting a role for miRNAs and their targets in morphological variation in the allopolyp...

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Bibliographic Details
Published in:The New phytologist 2010-04, Vol.186 (1), p.207-215
Main Authors: Lackey, Erika, Ng, Danny W.K, Chen, Z. Jeffrey
Format: Article
Language:English
Subjects:
AGO1
Allopolyploidy
Arabidopsis
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Argonaute Proteins
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Complementary DNA
DCL1
Developmental biology
Down regulation
Down-Regulation - genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes
heterosis
hybrid vigor
MicroRNA
MicroRNAs - metabolism
Phenotype
Plant genetics
Plants
Plants, Genetically Modified
Polyploidy
Ribonuclease III - genetics
Ribonuclease III - metabolism
RNA
RNA Interference
RNA, Small Interfering - metabolism
RNAi
small RNA
Transgenic plants
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Summary:Both natural and newly synthesized allopolyploids display nonadditive gene expression changes through genetic and epigenetic mechanisms. The nonadditively expressed genes include many microRNA (miRNA) targets, suggesting a role for miRNAs and their targets in morphological variation in the allopolyploids and their progenitors. We produced dominant-negative transgenic allotetraploid plants in Arabidopsis using RNA interference (RNAi) that downregulates the expression of miRNA biogenesis genes, including DCL1 and AGO1. RNAi of DCL1 and AGO1 led to dominant negative phenotypes and decreased accumulation of several miRNAs and a tasiRNA tested in the transgenic resynthesized allotetraploids. The results demonstrated that miRNA biogenesis genes are effectively downregulated in the resynthesized allotetraploids containing redundant homoeologous genes that are difficult to be manipulated by conventional mutation screens. These lines will be useful for studying the effects of miRNA biogenesis genes on growth and developmental variation in the allopolyploids.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2010.03187.x