Molecular determinants of the antitumor effects of trichostatin A in pancreatic cancer cells

AIM:To gain molecular insights into the action of the histone deacetylase inhibitor(HDACI) trichostatin-A(TSA) in pancreatic cancer(PC) cells.METHODS:Three PC cell lines,BxPC-3,AsPC-1 and CAPAN-1,were treated with various concentrations of TSA for def ined periods of time.DNA synthesis was assessed...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2010-04, Vol.16 (16), p.1970-1978
Main Authors: Emonds, Elisabeth, Fitzner, Brit, Jaster, Robert
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p21 - metabolism
Gene Expression Regulation, Neoplastic
Histone Deacetylase Inhibitors - pharmacology
Humans
Hydroxamic Acids - pharmacology
Imidazoles - pharmacology
Original
p38 Mitogen-Activated Protein Kinases - metabolism
Pancreatic Neoplasms - drug therapy
Pancreatic Neoplasms - metabolism
Phosphorylation
Proto-Oncogene Proteins p21(ras)
Pyridines - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
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Summary:AIM:To gain molecular insights into the action of the histone deacetylase inhibitor(HDACI) trichostatin-A(TSA) in pancreatic cancer(PC) cells.METHODS:Three PC cell lines,BxPC-3,AsPC-1 and CAPAN-1,were treated with various concentrations of TSA for def ined periods of time.DNA synthesis was assessed by measuring the incorporation of 5-bromo-2'deoxyuridine.Gene expression at the level of mRNA was quantif ied by real-time polymerase chain reaction.Expression and phosphorylation of proteins was monitored by imm...
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v16.i16.1970