Protein Kinase SnRK2.6 Mediates the Regulation of Sucrose Metabolism and Plant Growth in Arabidopsis

• Published in: Plant physiology (Bethesda) 2010-05, Vol.153 (1), p.99-113
• Main Authors: Zheng, Zhifu, Xu, Xiaoping, Crosley, Rodney A, Greenwalt, Scott A, Sun, Yuejin, Blakeslee, Beth, Wang, Lizhen, Ni, Weiting, Sopko, Megan S, Yao, Chenglin, Yau, Kerrm, Burton, Stephanie, Zhuang, Meibao, McCaskill, David G, Gachotte, Daniel, Thompson, Mark, Greene, Thomas W
• Format: Article
• Language: English
• Subjects: Abscisic Acid - metabolism; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological and medical sciences; CELL BIOLOGY AND SIGNAL TRANSDUCTION; Energy Metabolism; Fatty Acid Desaturases - metabolism; Fatty acids; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene expression regulation; Gene Expression Regulation, Plant; Genes; Germination; Hypersensitivity; Inactivation; Leaves; Mosaic Viruses; Plant growth; Plant growth regulators; Plant Leaves - metabolism; Plant Oils - metabolism; Plant physiology and development; Plants; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Promoter Regions, Genetic; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Seedlings; Seedlings - growth & development; Seeds - growth & development; Seeds - metabolism; Starches; Sucrose - metabolism; Sugars; Transgenic plants; Yeasts
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.109.150789

In higher plants, three subfamilies of sucrose nonfermenting-1 (Snf1)-related protein kinases have evolved. While the Snf1-related protein kinase 1 (SnRK1) subfamily has been shown to share pivotal roles with the orthologous yeast Snf1 and mammalian AMP-activated protein kinase in modulating energy and metabolic homeostasis, the functional significance of the two plant-specific subfamilies SnRK2 and SnRK3 in these critical processes is poorly understood. We show here that SnRK2.6, previously identified as crucial in the control of stomatal aperture by abscisic acid (ABA), has a broad expression pattern and participates in the regulation of plant primary metabolism. Inactivation of this gene reduced oil synthesis in Arabidopsis (Arabidopsis thaliana) seeds, whereas its overexpression increased Suc synthesis and fatty acid desaturation in the leaves. Notably, the metabolic alterations in the SnRK2.6 overexpressors were accompanied by amelioration of those physiological processes that require high levels of carbon and energy input, such as plant growth and seed production. However, the mechanisms underlying these functionalities could not be solely attributed to the role of SnRK2.6 as a positive regulator of ABA signaling, although we demonstrate that this kinase confers ABA hypersensitivity during seedling growth. Collectively, our results suggest that SnRK2.6 mediates hormonal and metabolic regulation of plant growth and development and that, besides the SnRK1 kinases, SnRK2.6 is also implicated in the regulation of metabolic homeostasis in plants.