Ultralow power trapping and fluorescence detection of single particles on an optofluidic chip

• Published in: Lab on a chip 2010-01, Vol.10 (2), p.189-194
• Main Authors: Kühn, S, Phillips, B S, Lunt, E J, Hawkins, A R, Schmidt, H
• Format: Article
• Language: English
• Subjects: Biopolymers - analysis; Electric Power Supplies; Energy Transfer; Equipment Design; Equipment Failure Analysis; Micro-Electrical-Mechanical Systems - instrumentation; Microfluidic Analytical Techniques - instrumentation; Micromanipulation - instrumentation; Optical Devices; Spectrometry, Fluorescence - instrumentation
• ISSN: 1473-0197; 1473-0189
• DOI: 10.1039/b915750f

The development of on-chip methods to manipulate particles is receiving rapidly increasing attention. All-optical traps offer numerous advantages, but are plagued by large required power levels on the order of hundreds of milliwatts and the inability to act exclusively on individual particles. Here, we demonstrate a fully integrated electro-optical trap for single particles with optical excitation power levels that are five orders of magnitude lower than in conventional optical force traps. The trap is based on spatio-temporal light modulation that is implemented using networks of antiresonant reflecting optical waveguides. We demonstrate the combination of on-chip trapping and fluorescence detection of single microorganisms by studying the photobleaching dynamics of stained DNA in E. coli bacteria. The favorable size scaling facilitates the trapping of single nanoparticles on integrated optofluidic chips.