Human Interleukin 7: Molecular Cloning and Growth Factor Activity on Human and Murine B-Lineage Cells

A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kD...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-01, Vol.86 (1), p.302-306
Main Authors: Goodwin, Raymond G., Lupton, Stephen, Schmierer, Ann, Hjerrild, Kathryn J., Jerzy, Rita, Clevenger, William, Gillis, Steve, Cosman, David, Namen, Anthony E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
B lymphocytes
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
Base Sequence
Biological and medical sciences
Biotechnology
Bone marrow cells
Cell growth
Cell lines
Cloning, Molecular
Complementary DNA
COS cells
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic technics
growth factors
Growth Substances - genetics
Humans
Interleukin-7
Interleukins - genetics
Interleukins - pharmacology
Interleukins - physiology
Lymphocyte Activation
lymphocytes B
lymphokines
man
Methods. Procedures. Technologies
Mice
Molecular cloning
Molecular Sequence Data
Recombinant Proteins - pharmacology
RNA
Sequence Homology, Nucleic Acid
Stromal cells
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Summary:A cDNA encoding biologically active human interleukin 7 was isolated by hybridization with the homologous murine clone. Nucleotide sequence analysis indicated that this cDNA was capable of encoding a protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4 kDa for the mature protein. Recombinant human interleukin 7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells. Analysis of RNA by blot hybridization demonstrated the presence of two size classes of interleukin 7 mRNA in human splenic and thymic tissue.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.1.302