Specific identification, grouping and differentiation of Campylobacter jejuni among thermophilic campylobacters using multiplex PCR

Campylobacter species such as C. jejuni and C. coli are recognized as major causes of acute gastroenteritis world-wide. Although C. jejuni and C. coli are usually non-pathogenic in birds and animals, they cause enteric disease in humans and the source of infection is often the consumption of contami...

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Bibliographic Details
Published in:Epidemiology and infection 2001-08, Vol.127 (1), p.1-5
Main Authors: KOROLIK, V., FRIENDSHIP, D. T., PEDURU-HEWA, T., ALFREDSON, D. A., FRY, B. N., COLOE, P. J.
Format: Article
Language:English
Subjects:
Animals
Australia
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Campylobacter
Campylobacter coli
Campylobacter jejuni
Campylobacter jejuni - classification
Campylobacter jejuni - genetics
Campylobacter jejuni - isolation & purification
Chickens
Cloning
DNA
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Fetuses
Fundamental and applied biological sciences. Psychology
Gastroenteritis
Genetic polymorphism
Genomics
Humans
Microbiology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Poultry
Product category rules
Salmonella
Sensitivity and Specificity
Sequencing
Techniques used in virology
Virology
Washing
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Summary:Campylobacter species such as C. jejuni and C. coli are recognized as major causes of acute gastroenteritis world-wide. Although C. jejuni and C. coli are usually non-pathogenic in birds and animals, they cause enteric disease in humans and the source of infection is often the consumption of contaminated foodstuffs. In this paper we report on the development and use of a multiplex PCR of C. jejuni genomic DNA which yielded a PCR product with a unique polymorphic site that can be used to quickly and accurately identify and group C. jejuni isolates from any source including DNA, cell culture, skin washings and faecal samples. The test is simple and sensitive and can detect purified DNA from a single bacterium 102 cells from crude lysates, 103 cells in seeded faeces and 120 cells/ml of washing of 1 cm2 skin fragments of chicken skin.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268801005763