Molecular characterization of Leptospira spp. strains isolated from small rodents in Croatia

We report the isolation and characterization of 16 Leptospira spp. strains isolated from small rodents captured in 11 different regions of inland Croatia. Large NotI and SgrAI restriction fragment allowed us to assign 10 isolates to the serovar istrica, 5 isolates to the serovar tsaratsovo and 1 iso...

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Bibliographic Details
Published in:Epidemiology and infection 2003-02, Vol.130 (1), p.159-166, Article S0950268802008026
Main Authors: TURK, N., MILAS, Z., MARGALETIC, J., STARESINA, V., SLAVICA, A., RIQUELME-SERTOUR, N., BELLENGER, E., BARANTON, G., POSTIC, D.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Collaboration
Croatia - epidemiology
DNA
DNA Primers
DNA, Ribosomal - blood
DNA, Ribosomal - genetics
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Kidneys
Leptospira
Leptospira - classification
Leptospira - genetics
Leptospira - isolation & purification
Leptospirosis
Leptospirosis - epidemiology
Leptospirosis - microbiology
Leptospirosis - veterinary
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Phylogenetics
Phylogeny
Ribosomal DNA
Rodent Diseases - epidemiology
Rodent Diseases - microbiology
Rodentia
Rodents
Sequencing
Vectors. Intermediate hosts
Veterinary colleges
Veterinary medicine
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Description
Summary:We report the isolation and characterization of 16 Leptospira spp. strains isolated from small rodents captured in 11 different regions of inland Croatia. Large NotI and SgrAI restriction fragment allowed us to assign 10 isolates to the serovar istrica, 5 isolates to the serovar tsaratsovo and 1 isolate to the serovar lora. The phylogenetic analysis conducted from the sequences of the first 330 bp from the 16S rDNA gene revealed that the strains belonged to three different species, L. borgpetersenii, L. kirschneri and L. interrogans. Carrier rates in eight rodent species varied from 0 to 71·4%. Mus musculus showed the highest infection level and confirmed its role as a major reservoir of the serogroup Sejroë. For the first time we reported the occurrence of serovars tsaratsovo and lora in Croatia.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268802008026