Transmission of two Australian strains of murine cytomegalovirus (MCMV) in enclosure populations of house mice (Mus domesticus)

To control plagues of free-living mice (Mus domesticus) in Australia, a recombinant murine cytomegalovirus (MCMV) expressing fertility proteins is being developed as an immunocontraceptive agent. Real-time quantitative PCR was used to monitor the transmission of two genetically variable field strain...

Full description

Saved in:
Bibliographic Details
Published in:Epidemiology and infection 2005-08, Vol.133 (4), p.701-710
Main Authors: FARROWAY, L. N., GORMAN, S., LAWSON, M. A., HARVEY, N. L., JONES, D. A., SHELLAM, G. R., SINGLETON, G. R.
Format: Article
Language:English
Subjects:
Animals
Animals, Wild
Antibodies
Base Sequence
Biological and medical sciences
Contraception, Immunologic - methods
Cytomegalovirus
Disease Models, Animal
Disease transmission
DNA
DNA, Viral - analysis
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Genomes
Herpesviridae Infections - prevention & control
Herpesviridae Infections - transmission
Herpesviridae Infections - veterinary
Infection Control
Infections
Lungs
Male
Mice
Microbiology
Miscellaneous
Molecular Sequence Data
Muromegalovirus - classification
Muromegalovirus - isolation & purification
Probability
Reverse Transcriptase Polymerase Chain Reaction
Salivary glands
Sensitivity and Specificity
Viral Vaccines - pharmacology
Virology
Virus Replication
Viruses
Western Australia
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To control plagues of free-living mice (Mus domesticus) in Australia, a recombinant murine cytomegalovirus (MCMV) expressing fertility proteins is being developed as an immunocontraceptive agent. Real-time quantitative PCR was used to monitor the transmission of two genetically variable field strains of MCMV through mouse populations after 25% of founding mice were infected with the N1 strain, followed by the G4 strain 6 weeks later. Pathogen-free wild-derived mice were released into outdoor enclosures located in northwestern Victoria (Australia). Of those mice not originally inoculated with virus, N1 DNA was detected in more than 80% of founder mice and a third of their offspring and similarly, G4 DNA was detected in 13% of founder mice and in 3% of their offspring. Thus, prior immunity to N1 did not prevent transmission of G4. This result is promising for successful transmission of an immunocontraceptive vaccine through Australian mouse populations where MCMV infection is endemic.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268805003717