Opa-typing can identify epidemiologically distinct subgroups within Neisseria gonorrhoeae multi-antigen sequence type (NG-MAST) clusters

A collection of 106 Neisseria gonorrhoeae ciprofloxacin-resistant isolates were typed using Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST). Opa-typing was performed on 74 isolates which had non-unique sequence types to determine if further discrimination could be achieved and if so wh...

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Bibliographic Details
Published in:Epidemiology and infection 2008-03, Vol.136 (3), p.417-420
Main Authors: MORRIS, A. K., PALMER, H. M., YOUNG, H.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Antigens, Bacterial - analysis
Bacteria
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Ciprofloxacin - pharmacology
DNA Primers
DNA, Bacterial - analysis
Drug Resistance, Microbial
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Gender discrimination
Gonorrhea - drug therapy
Gonorrhea - epidemiology
Gonorrhea - etiology
Gonorrhea - microbiology
Heterosexuality
Humans
Infections
Male
Microbiology
Miscellaneous
Neisseria gonorrhoeae
Neisseria gonorrhoeae - classification
Neisseria gonorrhoeae - drug effects
Neisseria gonorrhoeae - genetics
Neisseria gonorrhoeae - immunology
Neisseria gonorrhoeae - isolation & purification
Original Papers
Polymerase Chain Reaction
Scotland - epidemiology
Sexual orientation
Sexually transmitted diseases
Surveillance
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Summary:A collection of 106 Neisseria gonorrhoeae ciprofloxacin-resistant isolates were typed using Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST). Opa-typing was performed on 74 isolates which had non-unique sequence types to determine if further discrimination could be achieved and if so whether this had any epidemiological basis. The 74 isolates were separated into 12 sequence types and 20 opa-types (OT). Seven opa-type clusters were congruent with the sequence types and five sequence types could be subdivided by opa-typing. These results demonstrate that opa-typing can add a further level of discrimination compared with NG-MAST. The surveillance data for isolates in the largest sequence type cluster (ST 147) indicated that two major subdivisions OT 1 and OT 2 differed epidemiologically by patients' sexual preference and geographical location. ST 147 is a common strain that has been isolated in several countries since 1999; our results suggest that it has diverged into at least two epidemiologically discrete forms.
ISSN:0950-2688
1469-4409
DOI:10.1017/S0950268807008710