Postnatal testis development, Sertoli cell proliferation and number of different spermatogonial types in C57BL/6J mice made transiently hypo‐ and hyperthyroidic during the neonatal period

• Published in: Journal of anatomy 2010-05, Vol.216 (5), p.577-588
• Main Authors: Auharek, Sarah Alves, De França, Luiz Renato
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Animals, Newborn; Antithyroid Agents - metabolism; C57BL/6J mice; Cell Differentiation - drug effects; Cell Differentiation - physiology; Cell Proliferation; Hyperthyroidism - chemically induced; Hypothyroidism - chemically induced; Male; Mice; Mice, Inbred C57BL; Original; propylthiouracil (PTU); Propylthiouracil - metabolism; Sertoli cell; Sertoli Cells - cytology; Sertoli Cells - drug effects; Spermatogenesis - drug effects; Spermatogenesis - physiology; spermatogonia; Testis - cytology; Testis - drug effects; Testis - growth & development; testis development; Time Factors; triidothyronine (T3); Triiodothyronine - metabolism
• ISSN: 0021-8782; 1469-7580
• DOI: 10.1111/j.1469-7580.2010.01219.x

The role of thyroid hormones in testis structure and function has been fairly well studied in laboratory rodents. However, there are no comprehensive data in the literature for mice regarding the effects of transiently induced neonatal hypo‐ and hyperthyroidism on testis and spermatogonial cell development from birth to adulthood. Our goals were to evaluate the effects of propylthiouracil (PTU) and triidothyronine (T3) on Sertoli cell proliferation/differentiation and to correlate these events with the evolution of the spermatogenic process, tubular lumen formation, blood vessel volume density, and size and number of different spermatogonial types. Although Sertoli cell maturation was accelerated or delayed, respectively, in T3‐ and PTU‐treated mice, the pace of the germ cell maturation was only slightly altered before puberty and the period of Sertoli cell proliferation was apparently not affected by the treatments. However, compared with controls, the total number of Sertoli cells per testis from 10 days of age to adulthood was significantly increased and decreased in PTU‐ and T3‐treated mice, respectively. In comparison to all other spermatogonia, type A2 was the largest cell in all ages and groups investigated. The PTU‐treated mice had a significantly increased total number of undifferentiated spermatogonia as well as volume and percentage of vessels/capillaries, probably due to the higher number of Sertoli cells, particularly at 10 days of age. Taken together, our results suggest that neonatal hypothyroidism may be a valuable tool for studying spermatogonial biology as well as a means for providing more spermatogonial stem cells that could potentially be used for spermatogonial transplantation, thereby optimizing the efficiency of this technique when young mice are used as donors.