Identification and Characterization of a Promoter Cassette Conferring Adipocyte-Specific Gene Expression

The adipocyte-specific secretory molecule adiponectin has found widespread acceptance as a systemic marker that effectively integrates a number of signals associated with metabolic dysfunction at the level of adipose tissue. The widely used aP2 promoter cassette, which is frequently chosen to achiev...

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Bibliographic Details
Published in:Endocrinology (Philadelphia) 2010-06, Vol.151 (6), p.2933-2939
Main Authors: Wang, Zhao V, Deng, Yingfeng, Wang, Qiong A, Sun, Kai, Scherer, Philipp E
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - metabolism
Adiponectin
Adiponectin - genetics
Adipose tissue
Animal models
Animals
Biological and medical sciences
Cardiomyocytes
Cassettes
Cells, Cultured
Cre recombinase
Fundamental and applied biological sciences. Psychology
Gene expression
Genes
Humans
In vivo methods and tests
Integrases - metabolism
Introns - genetics
Macrophages
Mice
Mice, Transgenic
Promoter Regions, Genetic - genetics
Reverse Transcriptase Polymerase Chain Reaction
Transgenes
Transgenic animals
Transgenic mice
Vertebrates: endocrinology
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Summary:The adipocyte-specific secretory molecule adiponectin has found widespread acceptance as a systemic marker that effectively integrates a number of signals associated with metabolic dysfunction at the level of adipose tissue. The widely used aP2 promoter cassette, which is frequently chosen to achieve adipocyte-specific expression of transgenes, conveys transcription in cell types other than adipocytes, such as macrophages and cardiomyocytes. To improve our ability to drive transgene expression in a more adipocyte-specific way, we aimed to define the minimal promoter segment from the adiponectin genomic locus. We generated a series of transgenic animals in which the expression of reporter genes and Cre recombinase was driven by 2, 4.9, and 5.4 kb of adiponectin promoter sequences. We found that the 5.4-kb adiponectin promoter fragment is the most effective cassette conveying adipocyte-specific expression of target genes. We therefore define a novel promoter cassette that ensures adipocyte-specific expression of passenger genes and may be used in the generation of transgenic mouse models to study gene function in vivo. The promoter sequence of the adipocyte-specific adiponectin gene was characterized to obtain a generally useful cassette for transgenic expression of target genes and provide a validation in a number of different mouse models.
ISSN:0013-7227
1945-7170
DOI:10.1210/en.2010-0136