Histone H3 trimethylated at lysine 4 is enriched at probable transcription start sites in Trypanosoma brucei

The histone modification H3K4me3 is enriched at probable sites of transcription initiation in Trypanosoma brucei and co-localizes with H4K10ac and histone variants H2AZ and H2Bv. Recent studies have identified histone modifications and suggested a role for epigenetic gene regulation in Trypanosoma b...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2010-08, Vol.172 (2), p.141-144
Main Authors: Wright, Jessica R., Siegel, T. Nicolai, Cross, George A.M.
Format: Article
Language:English
Subjects:
Amino acids
Animals
BDF
bromodomain-containing proteins
ChIP
ChIP-seq
ChIP-sequencing
Chromatin Immunoprecipitation
Gene Expression Regulation
High-throughput DNA sequencing
Histone modification
Histones - metabolism
Lysine - metabolism
Methylation
Protein Binding
SSR
strand-switch regions
Transcription Initiation Site
Transcription regulation
transcription start sites
Trypanosoma brucei
Trypanosoma brucei brucei - genetics
Trypanosoma brucei brucei - metabolism
TSS
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Summary:The histone modification H3K4me3 is enriched at probable sites of transcription initiation in Trypanosoma brucei and co-localizes with H4K10ac and histone variants H2AZ and H2Bv. Recent studies have identified histone modifications and suggested a role for epigenetic gene regulation in Trypanosoma brucei. The histone modification H4K10ac and histone variants H2AZ and H2BV localize to probable sites of transcription initiation. Although all T. brucei histones have very evolutionarily divergent N-terminal tails, histone H3 shows conservation with other eukaryotic organisms in 6 of 8 amino acids encompassing lysine 4. Tri-methylation of H3K4 is generally associated with transcription. We therefore generated a specific antibody to T. brucei H3K4me3 and performed chromosome immunoprecipitation and high-throughput sequencing. We show that H3K4me3 is enriched at the start of polycistronic transcription units at divergent strand-switch regions and at other sites of RNA polymerase II transcription reinitiation. H3K4me3 largely co-localizes with H4K10ac, but with a skew towards the upstream side of the H4K10ac peak, suggesting that it is a component of specific nucleosomes that play a role in Pol II transcription initiation.
ISSN:0166-6851
1872-9428
DOI:10.1016/j.molbiopara.2010.03.013