Prostaglandin E2 is crucial in the response of podocytes to fluid flow shear stress

Podocytes play a key role in maintaining and modulating the filtration barrier of the glomerulus. Because of their location, podocytes are exposed to mechanical strain in the form of fluid flow shear stress (FFSS). Several human diseases are characterized by glomerular hyperfiltration, such as diabe...

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Bibliographic Details
Published in:Journal of cell communication and signaling 2010-06, Vol.4 (2), p.79-90
Main Authors: Srivastava, Tarak, McCarthy, Ellen T., Sharma, Ram, Cudmore, Patricia A., Sharma, Mukut, Johnson, Mark L., Bonewald, Lynda F.
Format: Article
Language:English
Subjects:
Actin
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cyclooxygenase
Life Sciences
Mechanical strain
Prostaglandin E2
Research Article
Shear stress
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Summary:Podocytes play a key role in maintaining and modulating the filtration barrier of the glomerulus. Because of their location, podocytes are exposed to mechanical strain in the form of fluid flow shear stress (FFSS). Several human diseases are characterized by glomerular hyperfiltration, such as diabetes mellitus and hypertension. The response of podocytes to FFSS at physiological or pathological levels is not known. We exposed cultured podocytes to FFSS, and studied changes in actin cytoskeleton, prostaglandin E 2 (PGE 2 ) production and expression of cyclooxygenase-1 and–2 (COX-1, COX-2). FFSS caused a reduction in transversal F-actin stress filaments and the appearance of cortical actin network in the early recovery period. Cells exhibited a pattern similar to control state by 24 h following FFSS without significant loss of podocytes or apoptosis. FFSS caused increased levels of PGE 2 as early as 30 min after onset of shear stress, levels that increased over time. PGE 2 production by podocytes at post-2 h and post-24 h was also significantly increased compared to control cells ( p  
ISSN:1873-9601
1873-961X
DOI:10.1007/s12079-010-0088-9