A Clomazone Immunoassay To Study the Environmental Fate of the Herbicide in Rice (Oryza sativa) Agriculture

The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzyme-linked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicid...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2010-04, Vol.58 (7), p.4367-4371
Main Authors: Carlomagno, Mariana, Mathó, Cecilia, Cantou, Guillermina, Sanborn, James R, Last, Jerold A, Hammock, Bruce D, Roel, Alvaro, González, David, González-Sapienza, Gualberto
Format: Article
Language:English
Subjects:
Agriculture
analytical methods
antibodies
Biological and medical sciences
Cereal and baking product industries
clomazone
Environmental Chemistry
environmental impact
Environmental Monitoring - methods
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
flood irrigation
Food industries
Fundamental and applied biological sciences. Psychology
hapten conjugation
haptens
herbicide residues
Herbicides - analysis
high performance liquid chromatography
Isoxazoles - analysis
Oryza - chemistry
Oryza - drug effects
Oryza sativa
Oxazolidinones - analysis
pesticide persistence
repeatability
rice
water pollution
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Summary:The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzyme-linked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0−20 ng/mL range in field samples, with excellent correlation with high-performance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf9043259