Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection

Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the flu...

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Bibliographic Details
Published in:Analytical biochemistry 2010-08, Vol.403 (1-2), p.123-125
Main Authors: Zhu, Aiping, Romero, Roberto, Petty, Howard R.
Format: Article
Language:English
Subjects:
Fluorescent Dyes - metabolism
Fluorometry - methods
Oxazines - metabolism
Pyruvic Acid - metabolism
Sensitivity and Specificity
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Summary:Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH dependent, which limits the sensitivity of the assay at pH 6.7. In this Note, we evaluate Invitrogen’s new dye, Amplex UltraRed, as a fluorogenic agent for pyruvate determination. Our results show that Amplex UltraRed improves the performance of the assay, providing brighter fluorescence and enhanced sensitivity.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2010.04.008