Crystallization and preliminary crystallographic studies of CorC, a magnesium-ion transporter

CorC is a magnesium transporter that is involved in the Mg2+‐efflux function of the CorA transporter system, an Mg2+ channel, from Shigella flexneri. Native CorC was purified and crystallized in the native form and in a ligand‐free form and diffraction data sets were collected to 2.9 and 3.4 Å resol...

Full description

Saved in:
Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2010-06, Vol.66 (6), p.681-683
Main Authors: Zhang, Ning, Ren, Xiaoming, Zhu, Deyu, Li, Defeng, Wang, Dacheng
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - chemistry
Adenosine Triphosphate - metabolism
Cation Transport Proteins - chemistry
Cation Transport Proteins - metabolism
Channels
CorA transporter system
CorC
Crystallization
Crystallization Communications
Crystallography
Crystallography, X-Ray
Crystals
Diffraction
Magnesium
magnesium-ion transporters
Protein Binding
Shigella flexneri
Transporter
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:CorC is a magnesium transporter that is involved in the Mg2+‐efflux function of the CorA transporter system, an Mg2+ channel, from Shigella flexneri. Native CorC was purified and crystallized in the native form and in a ligand‐free form and diffraction data sets were collected to 2.9 and 3.4 Å resolution, respectively. The native CorC crystals belonged to space group P212121, with unit‐cell parameters a = 64.31, b = 74.44, c = 132.78 Å. The ligand‐free CorC crystals belonged to space group P3121/P3221, with unit‐cell parameters a = b = 71.89, c = 125.96 Å. The CorC–ATP complex has also been crystallized and the crystals belonged to space group P2 or P21.
ISSN:1744-3091
1744-3091
DOI:10.1107/S1744309110012613