Multiscale Morphology of Organic Semiconductor Thin Films Controls the Adhesion and Viability of Human Neural Cells

• Published in: Biophysical journal 2010-06, Vol.98 (12), p.2804-2812
• Main Authors: Tonazzini, I., Bystrenova, E., Chelli, B., Greco, P., Stoliar, P., Calò, A., Lazar, A., Borgatti, F., D'Angelo, P., Martini, C., Biscarini, F.
• Format: Article
• Language: English
• Subjects: Adhesion; Astrocytes - cytology; Asymptotic properties; Biological Systems and Multicellular Dynamics; Biophysics; Brain; Cell Adhesion; Cell adhesion & migration; Cell Line, Tumor; Cell Proliferation; Cell Survival; Chemical Sciences; Correlation; Curvature; Engineering Sciences; Human; Humans; Life Sciences; Membranes; Microscopy; Microscopy, Atomic Force; Morphology; Naphthacenes - chemistry; Nervous System - cytology; Pentacene; Semiconductors; Thin films; Time Factors; Tumors
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2010.03.036

We investigate how multiscale morphology of functional thin films affects the in vitro behavior of human neural astrocytoma 1321N1 cells. Pentacene thin film morphology is precisely controlled by means of the film thickness, Θ (here expressed in monolayers (ML)). Fluorescence and atomic force microscopy allow us to correlate the shape, adhesion, and proliferation of cells to the morphological properties of pentacene films controlled by saturated roughness, σ, correlation length, ξ, and fractal dimension, df. At early incubation times, cell adhesion exhibits a transition from higher to lower values at Θ ≈ 10 ML. This is explained using a model of conformal adhesion of the cell membrane onto the growing pentacene islands. From the model fitting of the data, we show that the cell explores the surface with a deformation of the membrane whose minimum curvature radius is 90 (± 45) nm. The transition in the adhesion at ∼10 ML arises from the saturation of ξ accompanied by the monotonic increase of σ, which leads to a progressive decrease of the pentacene local radius of curvature and hence to the surface area accessible to the cell. Cell proliferation is also enhanced for Θ < 10 ML, and the optimum morphology parameter ranges for cell deployment and growth are σ ≤ 6 nm, ξ > 500 nm, and df > 2.45. The characteristic time of cell proliferation is τ ≈ 10 ± 2 h.